Immune checkpoint blockade : methods and protocols /

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Bibliographic Details
Group Author: Pico de Coaña, Yago (Editor)
Published: Humana Press,
Publisher Address: New York, NY :
Publication Dates: [2019]
Literature type: Book
Language: English
Series: Methods in molecular biology, 1913
Springer protocols
Subjects:
Carrier Form: xi, 225 pages : illustrations (some color), forms ; 26 cm.
Bibliography: Includes bibliographical references and index.
ISBN: 9781493989782 (hardcover) :
1493989782 (hardcover)
9781493989799 (ebook)
1493989790 (ebook)
Index Number: QR181
CLC: R392
Call Number: R392/I336-2
Contents: Double immunohistochemistry and digital image analysis /
Multispectral fluorescence imaging allows for distinctive topographic assessment and subclassification of tumor-infiltrating and surrounding immune cells /
Systems-level immune monitoring by mass cytometry /
Immune monitoring of cancer patients by multi-color flow cytometry /
High-throughput, fast, and sensitive immunopeptidomics sample processing for mass spectrometry /
Identification of immune modulatory miRNAs by miRNA enrichment via RNA affinity purification /
Expansion of tumor-infiltrating lymphocytes from melanoma tumors /
Establishment of two dimensional (2D) and three-dimensional (3D) melanoma primary cultures as a tool for in vitro drug resistance studies /
Assessment of antitumor T-cell responses by flow cytometry after coculture of tumor cells with autologous tumor-infiltrating lymphocytes /
Expansion and determination of antigen-reactive T cells by flow cytometry /
Assessing canonical and adaptive natural killer cell function in suppression assays in vitro /
Evaluating antibody-dependent cell-mediated cytotoxicity by chromium release assay /
Evaluating antibody-dependent cell-mediated cytotoxicity by flow cytometry /
Evaluation of breast cancer and melanoma metastasis in syngeneic mouse models /
Establishment of melanoma tumor xenograft using single cell line suspension and co-injection of patient-derived t cells in immune-deficient NSG mice /
Analysis of tissue-resident immune cells from mouse skin and lungs by flow cytometry /