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PCR applications : protocols for functional genomics /

PCR is the powerful technique used in molecular biology. This book examines the developments in this field. It discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. It covers quantitative PCR te...

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Bibliographic Details
Corporate Authors: Elsevier Science & Technology.
Group Author: Sninsky, John J.; Innis, Michael A.; Gelfand, David H.
Published: Academic Press,
Publisher Address: San Diego :
Publication Dates: 1999.
Literature type: eBook
Language: English
Subjects:
Polymerase chain reaction.
Gene amplification.
Polymerase chain reaction > Laboratory manuals.
Gene amplification > Laboratory manuals.
Genomics > Laboratory manuals.
Polymerase Chain Reaction > methods.
Genetic engineering.
Genomes
Polymerase chain reaction > Diagnostic use.
Re action en chai ne de la polyme rase.
Amplification ge nique.
Re action en chai ne de la polyme rase > Manuels de laboratoire.
Amplification ge nique > Manuels de laboratoire.
SCIENCE > Genetics & Genomics. > Genetics & Genomics.
Genomics.
Polymerase kettingreactie.
Genoom.
Electronic books.
Laboratory Manuals.
Online Access: http://www.sciencedirect.com/science/book/9780123721853
Summary: PCR is the powerful technique used in molecular biology. This book examines the developments in this field. It discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom. It covers quantitative PCR techniques, including the use of standards.
Carrier Form: 1 online resource (xviii, 566 pages, [3] pages of plates) : illustrations (some color)
Bibliography: Includes bibliographical references and index.
ISBN: 9780080919638
0080919634
Index Number: QP606
CLC: Q78-3
Contents: pt. 1. Key concepts for PCR-- Ch. 1. Optimization of PCR: conversations between Michael and David-- Ch. 2. The convergence of PCR, computers, and the human genome project: past, present, and future-- Ch. 3. Thermostable DNA polymerases: an update-- Ch. 4. Musings on microbial genomes-- Ch. 5. Statistical refinement of primer design parameters-- Ch. 6. Multiplex PCR: optimization guidelines-- Ch. 7. The use of immobilized mismatch binding protein for the optimization of PCR fidelity.
Ch. 8. A new generation of PCR instruments and nucleic acid concentration systems-- Ch. 9. Sequencing PCR products-- Ch. 10. Recent advances in high-temperature reverse transcription and PCR-- Ch. 11. Viral genotyping by a quantitative point mutation assay: application to HIV-1 drug resistance-- Ch. 12. In situ PCR-- pt. 2. Quantitative PCR-- Ch. 13. Standards for PCR assays-- Ch. 14. Rapid thermal cycling and PCR kinetics-- Ch. 15. Kinetics of competitive reverse transcriptase-PCR.
Ch. 16. Kinetic PCR analysis using a CCD camera and without using oligonucleotide probes-- Ch. 17. Quantification of telomerase activity using telomeric repeat amplification protocol-- pt. 3. Gene discovery-- Ch. 18. Differential display-- Ch. 19. Single-cell cDNA libraries-- Ch. 20. Whole cell assays-- Ch. 21. Screening differentially displayed PCR products by single-strand conformation polymorphism gels-- Ch. 22. Microsatellite protocols-- Ch. 23. Real-time quantitative PCR: uses in discovery research.
Ch. 24. Homology cloning: a molecular taxonomy of the archaea-- Ch. 25. Cloning mammalian homologs of drosophila genes-- Ch. 26. Cloning human homologs of yeast genes-- pt. 4. Genomics and expression profiling-- Ch. 27. Cellular transcriptome analysis using a kinetic PCR assay-- Ch. 28. Parallel analysis with biological chips-- Ch. 29. High-density cDNA grids for hybridization fingerprinting experiments-- Ch. 30. Comparative genomics hybridization-- Ch. 31. Genetic footprinting and functional maps of the yeast genome.
Ch. 32. Molecular analysis of microdissected tissue: laser capture microdissection-- Ch. 33. Amplified fragmant length polymorphism: studies on plant development-- Ch. 34. A florescent, multiplex solid-phase minisequencing method for genotyping cytochrome P450 genes-- Ch. 35. The Cleavase I enzyme for mutation and polymorphism scanning.

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