Screen LibraryFluorescence in situ hybridization (FISH) : application guide /
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| Group Author: | |
|---|---|
| Published: |
Springer,
|
| Publisher Address: | Berlin : |
| Publication Dates: | [2017] |
| Literature type: | Book |
| Language: | English |
| Edition: | Second edition. |
| Series: |
Springer Protocols Handbooks,
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| Subjects: | |
| Carrier Form: | xiii, 606 pages : illustrations (some color) ; 27 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9783662529577 3662529572 9783662529591 3662529599 |
| Index Number: | QP519 |
| CLC: | Q343-33 |
| Call Number: | Q343-33/F646/2nd ed. |
| Contents: |
Background / Microscopy and imaging / Optical filters and light sources for FISH / Classification of FISH probes / Generation of Paint Probes from Flow-Sorted and Microdissected Chromosomes / FISH-microdissection / Homemade locus-specific FISH probes : bacterial artificial chromosomes / Standard FISH procedure / Microwave treatment for better FISH results in a shorter time / FISH with and without COT1 DNA / Formamide-free fluorescence in situ hybridization (FISH) / One-Day Quick FISH / Telomere length measurement by FISH / RNA imaging in living cells / Replicative detargeting FISH (ReD-FISH) technique in studies of telomere replication / Pre- and postnatal diagnostics and research on peripheral blood, bone marrow, chorion, amniocytes, and fibroblasts / Application of FISH to previously GTG-banded and/or embedded cytogenetic slides / FISH in uncultivated amniocytes / Tumorcytogenetic diagnostics and research on blood and bone marrow smears or effusions / Characterization of mosaicism in different easy-to-acquire body tissues such as buccal smears, skin abrasions, hair root cells, or urine / Characterization of archived formalin-fixed/paraffin-embedded or cryofixed tissue, including nucleus extraction / FISH on sperm, spermatocytes and oocytes / Two- to three-color FISH / Multiplex FISH and spectral karyotyping / FISH banding techniques / cenM-FISH approaches / Heterochromatin-directed mFISH (HCM-FISH) / Subtelomeric and/or subcentromeric probe sets / Bar coding is back / Fluorescence in situ hybridization onto DNA fibres generated using molecular combing / Parental origin determination FISH : pod-FISH / Simultaneous fluorescence Immunostaining and FISH / RNA-directed FISH and immunostaining / Immunofluorescence staining for cytosine modifications like 5-Methylcytosine and its oxidative derivatives and FISH / CENP antibodies used additionally to FISH / Interphase FISH in diagnostics / Interphase FISH for detection of chromosomal mosaicism / Comet-FISH / Micronucleus FISH / Three-dimensional interphase analysis enabled by suspension FISH / Animal probes and ZOO-FISH / Three-dimensional immunofluorescence in situ hybridization in preimplantation mouse embryos / Fish-FISH : molecular cytogenetics in Fish species / FISH in lampbrush chromosomes / General protocol of FISH for insects / FISH in Drosophila / FISH on insect cells transfected with heterologous DNA / In situ hybridization to plant chromosomes / Yeast chromosome dynamics revealed by immuno FISH / FISHing for food microorganisms / Evaluation of polymicrobial involvement using fluorescence in situ hybridization (FISH) in clinical practice / Gold-FISH : in situ hybridization of microbial cells for combined fluorescence and scanning electron microscopy / Comparative genomic hybridization (CGH) and microdissection-based CGH (Micro-CGH) / |