Screen LibraryRecombinant protein production in yeast /
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| Group Author: | ; |
|---|---|
| Published: |
Humana Press,
|
| Publisher Address: | New York, NY : |
| Publication Dates: | [2019] |
| Literature type: | Book |
| Language: | English |
| Series: |
Methods in molecular biology,
1923 Springer protocols |
| Subjects: | |
| Carrier Form: | xii, 431 pages : illustrations (some color), forms ; 26 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781493990238 (hardback) : 1493990233 (hardback) 9781493990245 (ebook) 1493990241 (ebook) |
| Index Number: | TP248 |
| CLC: | Q591.2 |
| Call Number: | Q591.2/R311 |
| Contents: |
Established and Upcoming yeast expression systems / Detection and elimination of cellular bottlenecks in protein-producing yeasts / Pichia pastoris promoters / Selection of the optimal yeast host for the synthesis of recombinant enzymes / Screening and Selection of production strains : secretory protein expression and analysis in Hansenula polymorpha / Selection of heterologous protein-producing strains in Yarrowia lipolytica / High-throughput screening and selection of Pichia pastoris strains / Analyzing recombinant protein production in Pichia pastoris with targeted proteomics / CRISPR/Cas9-mediated homology-directed genome editing in Pichia pastoris / Tailoring N-glycan biosynthesis for production of therapeutic proteins in Saccharomyces cerevisiae / Improving the production of cofactor-containing proteins : production of human hemoglobin in yeast / Membrane protein production in yeast : modification of yeast membranes for human membrane protein production / Yeast surface display and cell sorting of antigen-binding Fc fragments / Guidelines for small-scale production and purification of hepatitis B surface antigen virus-like particles from recombinant Pichia pastoris / Efficient development of a mixed feed process for Pichia pastoris / Primary recovery of yeast culture supernatant for recombinant protein purification / Purification of recombinant glycoproteins from Pichia pastoris Culture Supernatants / LC-MS analysis of (glyco-)proteins of Pichia pastoris / Assessing the quality of recombinant products made in yeast / Quantification of recombinant products in yeast / |