Screen LibraryCalcium signaling protocols /
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| Group Author: | ; |
|---|---|
| Published: |
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| Literature type: | Book |
| Language: | English |
| Edition: | 3rd ed. |
| Series: |
Methods in molecular biology,
937 Springer protocols |
| Subjects: | |
| Item Description: | Previous ed.: 2006. |
| Carrier Form: | xi, 360 p. : ill. (some col.) ; 27 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781627030854 (hbk. : alk. paper) : 1627030859 (hbk. : alk. paper) 9781627030861 (ebk.) 1627030867 (ebk.) |
| Index Number: | QP535 |
| CLC: | Q582 |
| Call Number: | Q582/C144/3rd.ed. |
| Contents: |
Fluorescent measurement of [Ca²⁺]c : basic practical considerations / Measurement of [Ca²⁺]i in whole cell suspensions using Fura-2 / Confocal microscopy : theory and applications for cellular signaling / Ratiometric Ca²⁺ measurements using the FlexStation® scanning fluorometer / Measuring Ca²⁺ changes in multiwell format using the fluorometric imaging plate reader (FLIPR®) / Ratiometric [Ca²⁺]i measurements in adherent cell-lines using the NOVOstar microplate reader / Whole-cell patch-clamp recording of voltage-sensitive Ca²⁺ channel currents in single cells : heterologous expression systems and neurones / Combined calcium fluorescence recording with ionic currents in contractile cells / Measurement of phospholipase C by monitoring inositol phosphates using [³H]inositol labeling protocols in permeabilized cells / Single-cell imaging techniques for the real-time detection of IP₃ in live cells / Measurement of inositol(1,4,5)trisphosphate using a stereospecific radioreceptor mass assay / Measurement of [Ca²⁺]i in smooth muscle strips using front-surface fluorimetry / Calcium measurements from whole heart using Rhod-2 / Measurement of changes in endothelial and smooth muscle Ca²⁺ in pressurized arteries / Single cell and subcellular measurements of intracellular Ca²⁺ concentration / Simultaneous analysis of intracellular pH and Ca²⁺ from cell populations / Measurements of Ca²⁺ concentration with recombinant targeted luminescent probes / Chimeric G proteins in fluorimetric calcium assays : experience with opioid receptors / Compartmentalizing genetically encoded calcium sensors / Measurement of cytosolic-free Ca²⁺ in plant tissue / Measurement of Ca²⁺-ATPase activity (in PMCA and SERCA1) / |