Screen LibraryProteomics for drug discovery : methods and protocols /
This book reflects the drug development communitys interest in proteomics methods by providing researchers with the necessary knowledge for the implementation of a number of basic experimental and bioinformatics protocols in the drug discovery research laboratory. The focus of the collection is on s...
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| Group Author: | ; ; |
|---|---|
| Published: |
Humana Press,
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| Publisher Address: | New York, NY : |
| Publication Dates: | [2018] |
| Literature type: | Book |
| Language: | English |
| Series: |
Methods in molecular biology,
1647 Springer protocols |
| Subjects: | |
| Summary: |
This book reflects the drug development communitys interest in proteomics methods by providing researchers with the necessary knowledge for the implementation of a number of basic experimental and bioinformatics protocols in the drug discovery research laboratory. The focus of the collection is on select protocols and methodologies that address the analysis of post-translational modifications, targeted protein quantification, protein-protein, protein-lipid, or protein-ATP interactions, in silicolead identification, protein arrays, tissue and cell extract preparation, labeling, chemoproteomics, and drug efficacy assessment. Emphasis was placed not just on methods that describe large-scale protein analysis, but also on ones that are amenable to future implementation in a high-throughput format. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Proteomics for Drug Discovery: Methods and Protocolsaims to provide a deep knowledge of the fundamentals of disease biology as well the means that were developed for probing the complex biological milieu. |
| Carrier Form: | xi, 302 pages : illustrations (some color), forms ; 26 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781493972005 (hardback : alkaline paper) : 1493972006 (hardback : alkaline paper) 9781493972012 (eBook) 1493972014 (eBook) |
| Index Number: | RM301 |
| CLC: |
Q51 R918 |
| Call Number: | R918/P967 |
| Contents: |
Photoaffinity labeling-based chemoproteomics strategy for unbiased target deconvolution of small molecule drug candidates / Multiplexed liquid chromatography-multiple reaction monitoring mass spectrometry quantification of cancer signaling proteins / Monitoring dynamic changes of the cell surface glycoproteome by quantitative proteomics / High-resolution parallel reaction monitoring with electron transfer dissociation for middle-down proteomics : an application to study the quantitative changes induced by histone modifying enzyme inhibitors and activators / Preparation and immunoaffinity depletion of fresh frozen tissue homogenates for mass spectrometry-based proteomics in the context of drug target/biomarker discovery / Target identification using cell permeable and cleavable chloroalkane derivatized small molecules / Microfluidics-mass spectrometry of protein-carbohydrate interactions : applications to the development of therapeutics and Biomarker Discovery / Studying protein-protein interactions by biotin AP-tagged pulldown and LTQ-qrbitrap mass spectrometry / Post-translational modification profiling-functional proteomics for the analysis of immune regulation / Reverse phase protein arrays and drug discovery / Probing protein kinase-ATP interactions using a fluorescent ATP analog / Preparation of disease-related protein assemblies for single particle electron microscopy / Identification of lipid binding modulators using the protein-lipid overlay assay / Resazurin live cell assay : setup and fine-tuning for reliable cytotoxicity results / Exploring protein-protein interactions as drug targets for anti-cancer therapy with in silico workflows / Method to identify silent codon mutations that may alter peptide elongation kinetics and co-translational protein folding / In silico design of anticancer peptides / Docking and virtual screening in drug discovery / Bioinformatics resources for interpreting proteomics mass spectrometry data / |