Screen LibraryMechanobiology in Health and Disease /
Mechanobiology in Health and Disease brings together contributions from leading biologists, clinicians, physicists and engineers in one convenient volume, providing a unified source of information for researchers in this highly multidisciplinary area. Opening chapters provide essential background in...
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| Corporate Authors: | |
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| Group Author: | |
| Published: |
Elsevier Ltd. : Academic Press,
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| Publisher Address: | [Place of publication not identified] : |
| Publication Dates: | 2018. |
| Literature type: | eBook |
| Language: | English |
| Subjects: | |
| Online Access: |
https://www.sciencedirect.com/science/book/9780128129524 |
| Summary: |
Mechanobiology in Health and Disease brings together contributions from leading biologists, clinicians, physicists and engineers in one convenient volume, providing a unified source of information for researchers in this highly multidisciplinary area. Opening chapters provide essential background information on cell mechanotransduction and essential mechanobiology methods and techniques. Other sections focus on the study of mechanobiology in healthy systems, including bone, tendons, muscles, blood vessels, the heart and the skin, as well as mechanobiology studies of pregnancy. Final chapters address the nascent area of mechanobiology in disease, from the study of bone conditions, skin diseases and heart diseases to cancer. A discussion of future perspectives for research completes each chapter in the volume. i A This is a timely resource for both early-career and established researchers working on mechanobiology. |
| Carrier Form: | 1 online resource |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9780128129531 0128129530 |
| Index Number: | QP38 |
| CLC: | R33 |
| Contents: |
Front Cover; Mechanobiology in Health and Disease; Copyright; Contents; Contributors; Foreword to mechanobiology in health and disease; Preface; Acknowledgments; Chapter 1: Techniques for studying mechanobiology; 1. Introduction to Mechanobiology; 2. Animal Models and Tissue Engineering to Study Mechanobiology; 2.1. Analysis of a Single Cell; 2.1.1. Force application techniques to analyze a single cell; 2.1.1.1. Optical tweezers; 2.1.1.2. Atomic force microscopy; 2.1.1.3. Micropipette aspiration; 2.2. Cellular Interactions With Their Local Environment 2.2.1. Techniques to analyze cellular tractions2.2.1.1. Traction force microscopy; 2.2.1.2. Micropillar arrays; 2.3. Bioreactors to Mimic the in vivo Environment; 2.3.1. Types of bioreactors; 2.3.2. Future of bioreactors; 2.4. Animal Loading Models; 2.4.1. Noninvasive extrinsic skeletal loading models; 2.4.1.1. Tibial four-point bend model; 2.4.1.2. Ulnar compression model; 2.4.2. Embryonic animal models with an altered mechanical environment; 2.4.2.1. In ovo immobilization; 2.4.2.2. Mammalian models; 2.4.2.3. Zebrafish models; 2.5. Fluorescent Proteins (FPs) and Imaging Techniques 2.5.1. FPs as markers in mechanobiology2.5.2. Imaging technologies using FPs; 2.5.2.1. Live cell imaging; 2.5.2.2. Fluorescent resonance energy transfer (FRET); 2.5.2.3. Fluorescent recovery after photobleaching (FRAP); 2.5.2.4. Confocal and two-photon microscopy; 3. Molecular and Genetic Techniques to Study Mechanobiology; 3.1. Analysis of mRNA Expression; 3.1.1. Microarray analysis; 3.1.2. Transcriptomics: Total RNA and mRNA sequencing; 3.1.3. Quantitative real time PCR; 3.1.4. In situ hybridization; 3.2. Analysis at the Protein Level; 3.2.1. Immunohistochemistry; 3.2.2. Western blotting 3.2.3. ELISA3.3. Techniques for Editing Gene Function and Altering the Mechanical Environment; 3.3.1. In vitro mutagenesis-Mice; 3.3.2. CRISPR; 3.3.3. In ovo/ex ovo manipulation-Chick; 4. Computational Techniques in Mechanobiology; 4.1. Computational Modeling; 4.1.1. Computational fluid dynamics; 4.1.2. FE analysis; 4.1.3. Multiscale and multiphysics modeling; 4.2. Image Analysis; 4.2.1. Digital image correlation; 4.2.2. Particle image velocimetry; 5. Future Perspectives; References; Chapter 2: Cell geometric control of nuclear dynamics and its implications; 1. Introduction 1.1. Physical Link Between Nucleus and Cytoskeleton1.2. Microrheology of the Nucleus; 1.3. Boundary Conditions; 2. Nuclear Translational Motion; 2.1. Image Processing and Computational Methods; 2.2. Geometric Control of Nuclear Translation; 2.3. Role of Cytoskeleton in Nuclear Translation; 3. Nuclear Rotational Motion; 3.1. Image Processing and Computational Methods; 3.2. Cell Geometric Regulation of Nuclear Rotation; 3.3. Role of Cytoskeleton in Nuclear Rotation; 3.4. Implications of Nuclear Rotation in Cellular Functions; 4. Nuclear Envelope Fluctuations |