Screen LibraryMicroinjection : methods and protocols /
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| Group Author: | ; |
|---|---|
| Published: |
Humana Press,
|
| Publisher Address: | New York, NY : |
| Publication Dates: | [2019] |
| Literature type: | Book |
| Language: | English |
| Series: |
Methods in molecular biology,
1874 Springer protocols |
| Subjects: | |
| Carrier Form: | xiv, 540 pages : illustrations (some color), forms ; 26 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781493993987 (paperback) : 1493993984 (paperback) 9781493988303 (hardback) 1493988301 (hardback) 9781493988310 (eBook) 149398831X (eBook) |
| Index Number: | RM169 |
| CLC: | Q-33 |
| Call Number: | Q-33/M626-4 |
| Contents: |
Microinjection and micromanipulation : a historical perspective / Production of transgenic mice by pronuclear microinjection / Transgene recombineering in bacterial artificial chromosomes / Using TARGATT technology to generate site-specific transgenic mice / Generating genetically engineered mice Using a Spermatogonial Stem Cell-Mediated Method / Chimeric Mouse Generation by ES Cell Blastocyst microinjection and uterine transfer / Creating knockin alleles in mouse embryonic stem cells by CRISPR/Cas9-mediated homologous recombination without drug selection / Using CRISPR/Cas9 for gene knockout in immunodeficient NSG mice / Generation of CRISPR-edited rodents using a piezo-driven zygote injection technique / Delivery of CRISPR-Cas9 into mouse zygotes by electroporation / Generation of conditional knockout mice by sequential insertion of two loxP sites in Cis using CRISPR/Cas9 and single-stranded DNA oligonucleotides / Improvement of mouse cloning from any type of cell by nuclear injection / CARD method for simple vitrification of mouse oocytes : advantages and applications / CARD method for mouse sperm cryopreservation and in vitro fertilization using frozen-thawed sperm / Isolation and analysis of a genome-edited single-hepatocyte from a Cas9 transgenic mouse line / Microinjection and oviduct transfer procedures for rat model generation with CRISPR-Cas9 technology / Molecular aspects of zinc finger nucleases (ZFNs)-Mediated gene editing in rat embryos / Organ generation from knockedout rat blastocysts complemented with pluripotent stem cells / Generation of rabbit models by gene editing nucleases / Production of genetically engineered porcine embryos by handmade cloning / Electrofusion of 2-cell embryos for porcine tetraploid embryo production / Gene knockouts in goats using CRISPR/Cas9 system and somatic cell nuclear transfer / Generating goat mammary gland bioreactors for producing recombinant proteins by gene targeting / Production of transgenic chickens using cultured primordial germ cells and gonocytes / Using microinjection to generate genetically modified Caenorhabditis elegans by CRISPR/Cas9 editing / Microinjection in zebrafish for genome editing and functional studies / Microinjection of marine fish eggs / Generating gene knockout Oryzias latipes and rice field eel using TALENs method / Functional studies of transcriptional cofactors via microinjection-mediated gene editing in xenopus / Microinjection of live mammalian cells : a delivery method that provides added versatility to the study of cellular function / |