Screen LibraryMetagenomics : methods and protocols /
This second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by the construction and screen...
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| Group Author: | ; |
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| Published: |
Humana Press ; Springer,
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| Publisher Address: | New York, NY : |
| Publication Dates: | [2017] |
| Literature type: | Book |
| Language: | English |
| Edition: | Second edition. |
| Series: |
Methods in molecular biology,
1539 Springer protocols |
| Subjects: | |
| Summary: |
This second edition explores up-to-date tools in various function-based technologies currently used in metagenomics. The chapters in this book discuss all of the working steps involved in these technologies, such as: DNA isolation from soils and marine samples followed by the construction and screening of libraries for diverse enzymes and biomolecules; current methods used to isolate DNA and construct large- and small-insert libraries from terrestrial and marine habitats; methods for establishing metagenome libraries in none-E.coli hosts; new molecular tools used for function-driven mining o |
| Carrier Form: | xi, 303 pages : illustrations (chiefly color) ; 27 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781493966899 1493966898 9781493966912 149396691X |
| Index Number: | QH434 |
| CLC: | Q343.1 |
| Call Number: | Q343.1/S915-1/2nd ed. |
| Contents: |
Construction of small-insert and large-insert metagenomic libraries / Extraction of total DNA and RNA from marine filter samples and generation of a cDNA as universal template for marker gene studies / Construction and screening of marine metagenomic large insert libraries / Constructing and screening a metagenomic library of a cold and alkaline extreme environment / |