Screen LibraryLong non-coding RNAs : methods and protocols /
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| Group Author: | ; |
|---|---|
| Published: |
Humana Press,
|
| Publisher Address: | New York : |
| Publication Dates: | [2016] |
| Literature type: | Book |
| Language: | English |
| Series: |
Methods in molecular biology,
1402 Springer protocols, |
| Subjects: | |
| Carrier Form: | xii, 298 pages : illustrations (some color) ; 27 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781493933761 1493933760 |
| Index Number: | QP623 |
| CLC: | Q522 |
| Call Number: | Q522/L849 |
| Contents: |
LncRNA pulldown combined with mass spectrometry to identify the novel LncRNA-associated proteins / Cross-linking immunoprecipitation and qPCR (CLIP-qPCR) analysis to map interactions between long noncoding RNAs and RNA-binding proteins / Characterization of long noncoding RNA-associated proteins by RNA-immunoprecipitation / Isolation of protein complexes associated with long noncoding RNAs / Profiling long noncoding RNA expression using custom-designed microarray / Long noncoding RNA expression profiling using arraystar LncRNA microarrays / Nuclear RNA isolation and sequencing / Targeted LncRNA sequencing with the SeqCap RNA enrichment system / ChIP-Seq : library preparation and sequencing / Stellaris® RNA fluorescence in situ hybridization for the simultaneous detection of immature and mature long noncoding RNAs in adherent cells / Simultaneous RNA-DNA FISH / Visualizing long noncoding RNAs on chromatin / Non-isotopic method for in situ LncRNA visualization and quantitation / Detection of long noncoding RNA expression by nonradioactive northern blots / Assessment of in vivo SiRNA delivery in cancer mouse models / Targeting long noncoding RNA with antisense oligonucleotide technology as cancer therapeutics / Characterization of circular RNAs / Methods for characterization of alternative RNA splicing / NONCODEv4 : annotation of noncoding RNAs with emphasis on long noncoding RNAs / Computational analysis of LncRNA from cDNA sequences / Analyzing MiRNA-LncRNA interactions / Long noncoding RNAs : an overview / |