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Aqueous size-exclusion chromatography /

The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polym...

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Bibliographic Details
Corporate Authors: Elsevier Science & Technology.
Group Author: Dubin, Paul. (Editor)
Published: Elsevier ; Distributors for the U.S. and Canada, Elsevier Science Pub. Co.,
Publisher Address: Amsterdam ; New York : New York, NY, U.S.A. :
Publication Dates: 1988.
Literature type: eBook
Language: English
Series: Journal of chromatography library ; v. 40
Subjects:
Gel permeation chromatography.
Chromatographie sur gel.
SCIENCE > Analytic. > Analytic.
Hydrofobe interactie chromatografie.
Biopolymeren.
Poreuze media.
Silica.
Polymers > Chromatography
Electronic books.
Online Access: http://www.sciencedirect.com/science/bookseries/03014770/40
Summary: The rapid development of new packings for aqueous size-exclusion chromatography has revolutionized this field. High resolution non-adsorptive columns now make possible the efficient separation of proteins and the rapid and precise determination of the molecular weight distribution of synthetic polymers. This technology is also being applied to the separation of small ions, the characterization of associating systems, and the measurement of branching. At the same time, fundamental studies are elucidating the mechanisms of the various chromatographicprocesses. These developments in principles and applications are assembled for the first time in this book. Fundamental issues are dealt with: the roles of pore structure and macromolecular dimensions, hydrophobic and electrostatic effects, and the determination and control of column efficiency. High-performance packings based on derivatized silica are reviewed in detail. Special techniques are thoroughly described, including SEC/LALLS, inverse exclusion chromatography, and frontal zone chromatography. Attention is focussed on special applications of size-exclusion methods, such as the characterization of micelles, separations of inorganic ions, and Hummel-Dreyer and related methods for equilibrium systems. Protein chromatography is dealt with in both dedicated sections and throughout the book as a whole. This is a particularly comprehensive and authoritative work - all the contributions review broad topics of general significance and the authors are of high repute. The material will be of special value for the characterization of synthetic water-soluble polymers, especially polyelectrolytes. Biochemists will find fundamental and practical guidance on protein separations. Researchers confronted with solutes that exhibit complex chromatographic behavior, such as humic acids, aggregating proteins, and micelles should find the contents of this volume illuminating.
Carrier Form: 1 online resource (xviii, 453 pages) : illustrations.
Bibliography: Includes bibliographical references and index.
ISBN: 9780444429575
0444429573
9780080858449
0080858449
Index Number: QD272
CLC: O658.1
Contents: Chapter 9. Rigid polymer gels for SEC and their application to biopolymersPart IV: Biopolymers; Chapter 10. Biopolymers. I. Protein chromatography in denaturing and non-denaturing solvents; Chapter 11. Biopolymers. II. Serum lipoproteins; Chapter 12. Application of SEC/LALLS to biopolymer assemblies; Part V: Associating Systems; Chapter 13. Measurement of equilibrium constants by exclusion methods; Chapter 14. Frontal.

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