In vitro biological systems /

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Bibliographic Details
Corporate Authors: Elsevier Science & Technology.
Group Author: Tyson, Charles A.; Frazier, John M.
Published: Academic Press,
Publisher Address: San Diego :
Publication Dates: 1993.
Literature type: eBook
Language: English
Series: Methods in toxicology, v. 1, pt. A
Subjects:
Online Access: http://www.sciencedirect.com/science/book/9780124612013
Carrier Form: 1 online resource (xxi, 568 pages, [1] pages of plates) : illustrations.
Bibliography: Includes bibliographical references and index.
Index Number: QH585
CLC: R99
Contents: Neural and neuromuscular systems -- Hippocampal slices -- Organotypic neural cultures -- Reaggregate cultures for neurotoxicological studies -- Cytotoxicity in murine neocortical cell culture -- Cultures of adult trigeminal ganglion neurons -- Adrenomedullary chromaffin cells for toxicological studies -- PC-12 cells -- Ocular system -- Corneal epithelial and endothelial cell culture -- Isolation and culture of retinal epithelium -- Respiratory system -- Isolation and culture of type II alveolar epithelial cells -- Isolation of nonciliated bronchiolar (Clara) epithelial cells from mouse lung -- Rat tracheal epithelial cell cultures for studies in toxicology and carcinogenicity -- Cardiovascular system -- Preparation of primary cultures of postnatal rat myocardial cells for toxicological studies -- Aortic endothelial and smooth muscle cell cultures -- Vessel cylinders -- Gastrointestinal system -- Gastric mucosal cell culture for toxicological study -- Small intestinal enterocytes -- Maintenance and characterization of an organ culture system for colonic mucosa.
(cont) Liver slices -- Precision-cut rat liver slices in dynamic organ culture for structure-toxicity studies -- Liver: hepatocytes -- Isolation of hepatocytes by collagenase perfusion -- Preparation of primary monolayer cultures of postnatal rat liver cells for hepatotoxic assessment of xenobiotics -- Isolation and culture of hepatocytes from different laboratory species -- Isolation of human hepatocytes by biopsy perfusion methods -- Human hepatocyte cultures -- Liver: other cell systems -- Hepatocyte and spleen cell systems -- Isolation and culture of hepatic nonparenchymal cells -- Proliferating lines of rat liver epithelial cells -- Isolation and culture of liver epithelial cells from carcinogen-treated rats -- Kidney: proximal tubule fragments -- Renal proximal tubule preparations isolated from rat or rabbit kidneys without the use of collagenase -- Suspension culture of rabbit renal proximal tubules -- Perfusion method for isolation of renal proximal tubules from mammalian species -- Isolation and maintenance of milligram quantities of rabbit renal proximal straight and convoluted tubules.
(cont) Kidney cell culture -- Rabbit cortical epithelial cells -- Culture of rat kidney proximal tubule epithelial cells in defined medium -- Primary culture of rat renal medullary interstitial cells -- Purification of renal cortical cell populations by percoll density-gradient centrifugation -- Renal cell lines derived from rabbit and pig kidney -- Reproductive and developmental systems -- Whole embryo culture: organogenesis of rodent embryos in vitro -- Preparation and use of sertoli-germ cell cocultures from 28-day-old rats -- Immune system -- Isolation and in vitro culture of mononuclear phagocytes -- T-lymphocyte assays -- B lymphocytes: comparison of murine and human B-cell immunocompetence -- Bone marrow stromal cell cultures -- Skin -- Penetration studies with excised human and animal skin -- Porcine skin flaps -- Keratinocytes.