Screen LibraryRNA detection : methods and protocols /
RNA molecules interact with proteins to perform a variety of functions in living cells. The binding partners of many RNAs, in particular the newly discovered class of long noncoding RNAs (lncRNAs), remain largely unknown. RNA antisense purification coupled with mass spectrometry (RAP-MS) is a method...
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| Group Author: | |
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| Published: |
Humana Press,
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| Publisher Address: | New York, NY : |
| Publication Dates: | [2018] |
| Literature type: | Book |
| Language: | English |
| Series: |
Methods in molecular biology,
volume 1649 Springer Protocols |
| Subjects: | |
| Summary: |
RNA molecules interact with proteins to perform a variety of functions in living cells. The binding partners of many RNAs, in particular the newly discovered class of long noncoding RNAs (lncRNAs), remain largely unknown. RNA antisense purification coupled with mass spectrometry (RAP-MS) is a method that enables the identification of direct and specific protein interaction partners of a specific RNA molecule. Because RAP-MS uses direct RNA-protein cross-linking methods coupled along with highly denaturing purification conditions, RAP-MS provides a short list of high confidence protein intera |
| Carrier Form: | xiv, 492 pages : illustrations (some color) ; 26 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781493972128 149397212X |
| Index Number: | QH506 |
| CLC: | Q522-33 |
| Call Number: | Q522-33/R627 |