Screen LibraryQuantitative imaging in cell biology /
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| Group Author: | ; |
|---|---|
| Published: |
Elsevier/AP,
|
| Publisher Address: | Amsterdam : |
| Publication Dates: | 2014. |
| Literature type: | Book |
| Language: | English |
| Edition: | First edition. |
| Series: |
Methods in cell biology,
volume 123 |
| Subjects: | |
| Carrier Form: | xx, 568 pages, [20] pages of plates : illustrations (some color) ; 24 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9780124201385 : 0124201385 |
| Index Number: | R857 |
| CLC: | Q24 |
| Call Number: | Q24/Q17 |
| Contents: |
Machine generated contents note: ch. 1 Concepts in Quantitative Fluorescence Microscopy / Torsten Wittmann -- 1.1.Accurate and Precise Quantitation -- 1.2.Signal, Background, and Noise -- 1.3.Optical Resolution: The Point Spread Function -- 1.4.Choice of Imaging Modality -- 1.5.Sampling: Spatial and Temporal -- 1.6.Postacquisition Corrections -- 1.7.Making Compromises -- 1.8.Communicating Your Results -- Acknowledgment -- References -- ch. 2 Practical Considerations of Objective Lenses for Application in Cell Biology / Michael W. Davidson -- Introduction -- 2.1.Optical Aberrations -- 2.2.Types of Objective Lenses -- 2.3.Objective Lens Nomenclature -- 2.4.Optical Transmission and Image Intensity -- 2.5.Coverslips, Immersion Media, and Induced Aberration -- 2.6.Considerations for Specialized Techniques -- 2.7.Care and Cleaning of Optics -- Conclusions -- References -- ch. 3 Assessing Camera Performance for Quantitative Microscopy / Jennifer C. Waters -- Contents note continued: 3.1.Introduction to Digital Cameras for Quantitative Fluorescence Microscopy -- 3.2.Camera Parameters -- 3.3.Testing Camera Performance: The Photon Transfer Curve -- References -- ch. 4 A Practical Guide to Microscope Care and Maintenance / Jennifer C. Waters -- Introduction -- 4.1.Cleaning -- 4.2.Maintenance and Testing -- 4.3.Considerations for New System Installation -- Acknowledgments -- References -- ch. 5 Fluorescence Live Cell Imaging / Torsten Wittmann -- 5.1.Fluorescence Microscopy Basics -- 5.2.The Live Cell Imaging Microscope -- 5.3.Microscope Environmental Control -- 5.4.Fluorescent Proteins -- 5.5.Other Fluorescent Probes -- Conclusion -- Acknowledgments -- References -- ch. 6 Fluorescent Proteins for Quantitative Microscopy: Important Properties and Practical Evaluation / Nathan Christopher Shaner -- 6.1.Optical and Physical Properties Important for Quantitative Imaging -- 6.2.Physical Basis for Fluorescent Protein Properties -- Contents note continued: 6.3.The Complexities of Photostability -- 6.4.Evaluation of Fluorescent Protein Performance in Vivo -- Conclusion -- References -- ch. 7 Quantitative Confocal Microscopy: Beyond a Pretty Picture / Richard W. Cole -- 7.1.The Classic Confocal: Blocking Out the Blur -- 7.2.You Call that Quantitative? -- 7.3.Interaction and Dynamics -- 7.4.Controls: Who Needs Them? -- 7.5.Protocols -- Conclusions -- References -- ch. 8 Assessing and Benchmarking Multiphoton Microscopes for Biologists / Matthew F. Krummel -- Introduction: Practical Quantitative 2P Benchmarking -- 8.1.Part I: Benchmarking Inputs -- 8.2.Part II: Benchmarking Outputs -- 8.3.Troubleshooting/Optimizing -- 8.4.A Recipe for Purchasing Decisions -- Conclusion -- Acknowledgments -- References -- ch. 9 Spinning-disk Confocal Microscopy: Present Technology and Future Trends / Mark Browne -- 9.1.Principle of Operation -- 9.2.Strengths and Weaknesses -- 9.3.Improvements in Light Sources -- Contents note continued: 9.4.Improvements in Illumination -- 9.5.Improvements in Optical Sectioning and FOV -- 9.6.New Detectors -- 9.7.A Look into the Future -- References -- ch. 10 Quantitative Deconvolution Microscopy / Paul C. Goodwin -- Introduction -- 10.1.The Point-spread Function -- 10.2.Deconvolution Microscopy -- 10.3.Results -- Conclusion -- References -- ch. 11 Light Sheet Microscopy / Jan-Huisken -- Introduction -- 11.1.Principle of Light Sheet Microscopy -- 11.2.Implementations of Light Sheet Microscopy -- 11.3.Mounting a Specimen for Light Sheet Microscopy -- 11.4.Acquiring Data -- 11.5.Handling of Light Sheet Microscopy Data -- References -- ch. 12 DNA Curtains: Novel Tools for Imaging Protein--Nucleic Acid Interactions at the Single-Molecule Level / Eric C. Greene -- Introduction -- 12.1.Overview of TIRFM -- 12.2.Flow Cell Assembly -- 12.3.Importance of the Lipid Bilayer -- 12.4.Barriers to Lipid Diffusion -- 12.5.Different Types of DNA Curtains -- Contents note continued: 12.6.Using DNA Curtains to Visualize Protein-DNA Interactions -- 12.7.Future Perspectives -- Acknowledgments -- References -- ch. 13 Nanoscale Cellular Imaging with Scanning Angle Interference Microscopy / Matthew Paszek -- Introduction -- 13.1.Experimental Methods and Instrumentation -- 13.2.Image Analysis and Reconstruction -- Conclusion -- Acknowledgments -- References -- ch. 14 Localization Microscopy in Yeast / Jonas Ries -- Introduction -- 14.1.Preparing the Yeast Strain -- 14.2.Considerations for the Choice of a Labeling Strategy -- 14.3.Preparing the Sample -- 14.4.Image Acquisition -- 14.5.Results -- Summary -- Acknowledgments -- References -- ch. 15 Imaging Cellular Ultrastructure by PALM, iPALM, and Correlative iPALM-EM / Pakorn Kanchanawong -- Introduction -- 15.1.Principles -- 15.2.Methods -- 15.3.Future Directions -- Acknowledgments -- References -- ch. 16 Seeing More with Structured Illumination Microscopy / Reto Fiolka -- Introduction -- Contents note continued: 16.1.Theory of Structured Illumination -- 16.2.3D SIM -- 16.3.SIM Imaging Examples -- 16.4.Practical Considerations and Potential Pitfalls -- 16.5.Discussion -- References -- ch. 17 Structured Illumination Superresolution Imaging of the Cytoskeleton / Ulrike Engel -- Introduction -- 17.1.Instrumentation for SIM Imaging -- 17.2.Sample Preparation -- 17.3.Minimizing Spherical Aberration -- 17.4.Multichannel SIM -- 17.5.Live Imaging with SIM -- Acknowledgments -- References -- ch. 18 Analysis of Focal Adhesion Turnover: A Quantitative Live-Cell Imaging Example / Torsten Wittmann -- Introduction to Focal Adhesion Dynamics -- 18.1.FA Turnover Analysis -- Acknowledgments -- References -- ch. 19 Determining Absolute Protein Numbers by Quantitative Fluorescence Microscopy / Kerry Bloom -- Introduction -- 19.1.Methods for Counting Molecules -- 19.2.Protocol for Counting Molecules by Ratiometric Comparison of Fluorescence Intensity -- Conclusions -- References -- Contents note continued: ch. 20 High-Resolution Traction Force Microscopy / Clare M. Waterman -- Introduction -- 20.1.Materials -- 20.2.Methods -- References -- ch. 21 Experimenters' Guide to Colocalization Studies: Finding a Way Through Indicators and Quantifiers, in Practice / Susanne Bolte -- Introduction -- 21.1.An Overview of Colocalization Approaches -- Conclusion -- References -- ch. 22 User-Friendly Tools for Quantifying the Dynamics of Cellular Morphology and Intracellular Protein Clusters / Klaus M. Hahn -- Introduction -- 22.1.Automated Classification of Cell Motion Types -- 22.2.GUI for Morphodynamics Classification and Ready Representation of Changes in Cell Behavior Over Time -- 22.3.Results of Morphodynamics Classification -- 22.4.Geometry-based Segmentation of Cells in Clusters -- 22.5.GUI for Cell Segmentation and Quantification of Protein Clusters -- 22.6.Results for Quantifying Protein Clusters -- 22.7.Discussion -- Acknowledgments -- References -- Contents note continued: ch. 23 Ratiometric Imaging of pH Probes / Diane L. Barber -- Introduction -- 23.1.Currently Used Ratiometric pH Probes -- 23.2.Applications -- 23.3.Protocols -- Acknowledgments -- References -- ch. 24 Toward Quantitative Fluorescence Microscopy with DNA Origami Nanorulers / Philip Tinnefeld -- Introduction -- 24.1.The Principle of DNA Origami -- 24.2.Functionalizing DNA Origami Structures -- 24.3.DNA Origami as Fluorescence Microscopy Nanorulers -- 24.4.Brightness References Based on DNA Origami -- 24.5.Applications of DNA Origami Nanorulers for Visualizing Resolution -- 24.6.How to Choose an Appropriate Nanoruler for a Given Application -- References -- ch. 25 Imaging and Physically Probing Kinetochores in Live Dividing Cells / Sophie Dumont -- Introduction -- 25.1.Spindle Compression to Image and Perturb Kinetochores -- 25.2.Imaging Kinetochore Dynamics at Subpixel Resolution Via Two-Color Reporter Probes -- Conclusion and Outlook -- Acknowledgments -- Contents note continued: References -- ch. 26 Adaptive Fluorescence Microscopy by Online Feedback Image Analysis / Rainer Pepperkok -- Introduction -- 26.1.Requirements for Adaptive Feedback Microscopy -- 26.2.Selected Applications -- Acknowledgments -- References -- ch. 27 Open-Source Solutions for SPIMage Processing / Pavel Tomancak -- Introduction -- 27.1.Prerequisites -- 27.2.Overview of the SPIM Image-Processing Pipeline -- 27.3.Bead-Based Registration -- 27.4.Multiview Fusion -- 27.5.Processing on a High-Performance Cluster -- 27.6.Future Applications -- References -- ch. 28 Second-Harmonic Generation Imaging of Cancer / Kevin W. Eliceiri -- Introduction -- 28.1.SHG Physical and Chemical Background -- 28.2.SHG Instrumentation -- 28.3.Collagen Structure as a Biomarker -- 28.4.SHG in Cancer Research -- 28.5.Quantitative Analysis of SHG Images -- Conclusion -- References. |