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Methods in enzymology. Volume five hundred and eighty two, Single-molecule enzymology : nanomechanical manipulation and hybrid methods /

This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion.

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Bibliographic Details
Group Author: Spies, Maria; Chemla, Yann R
Published: Academic Press is an imprint of Elsevier,
Publisher Address: Cambridge, MA :
Publication Dates: 2017.
Literature type: Book
Language: English
Edition: First edition.
Series: Methods in enzymology, volume 582
Subjects:
Enzymology
Enzymes > Research > Methodology
Enzyme kinetics
Atomic force microscopy
Fluorescence spectroscopy
Fluorescence microscopy
Molecular biology
Nanostructured materials
Summary: This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion.
Carrier Form: xvi, 467 pages : illustrations (some color) ; 24 cm.
Bibliography: Includes bibliographical references and indexes.
ISBN: 9780128093108 (hardback) :
0128093102 (hardback)
Index Number: QP601
CLC: Q55-3
Call Number: Q55-3/M592/v.582
Contents: 1. How to Measure Load-Dependent Kinetics of Individual Motor Molecules Without a Force-Clamp / J. Sung, K.I. Mortensen, J.A. Spudich, H. Flyvbjerg -- 2. Studying the Mechanochemistry of Processive Cytoskeletal Motors With an Optical Trap / V. Belyy, A. Yildiz -- 3. Single-Molecule Optical-Trapping Techniques to Study Molecular Mechanisms of a Replisome / B. Sun, M.D. Wang 4. Recent Advances in Biological Single-Molecule Applications of Optical Tweezers and Fluorescence Microscopy / M. Hashemi Shabestari, A.E.C. Meijering, W.H. Roos, G.J.L. Wuite, E.J.G. Peterman -- 5. Direct Visualization o

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