Macrophages : methods and protocols /
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Group Author: | |
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Published: |
Humana Press ; Springer,
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Publisher Address: | New York, NY : |
Publication Dates: | [2018] |
Literature type: | Book |
Language: | English |
Series: |
Methods in molecular biology (Clifton, N.J.) ; volume 1784. 1064-3745 |
Subjects: | |
Carrier Form: | xii, 278 pages : illustrations (some color) ; 26 cm. |
Bibliography: | Includes bibliographical references and index. |
ISBN: |
9781493978366 1493978365 |
Index Number: | QR185 |
CLC: | Q939.48 |
Call Number: | Q939.48/M174-3 |
Contents: |
Culture of human monocyte-derived macrophages / Simple multistep protocol for differentiating human induced pluripotent stem cells into functional macrophages / Growing murine bone marrow-derived macrophages / Immortalization of murine bone marrow-derived macrophages / Isolation and identification of murine serous cavity macrophages / Isolation and identification of interstitial macrophages from the lungs using different digestion enzymes and staining strategies / Isolation and phenotyping of adult mouse microglial cells / Isolation and phenotyping of bone marrow macrophages / Isolation and phenotyping of intestinal macrophages / Phenotyping tumor-associated macrophages / Activating murine macrophages In Vitro / Polarizing macrophages in vitro / Viral replication assay in bone marrow-derived macrophages / Macrophage bactericidal assays / Quantitative phagocytosis assays in primary and cultured macrophages / Observing frustrated phagocytosis and phagosome formation and closure using total internal reflection fluorescence microscopy (TIRFM) / Chromatin immunoprecipitation in macrophages / Transfecting macrophages / In vitro migration assays / Cytokine-induced acute inflammatory monoarticular arthritis / Characterizing activation, proliferation, and ontogeny of murine macrophages in parasitic helminth infections / Genetic models of macrophage depletion / Depleting macrophages in vivo with clodronate-liposomes / Cre driver mice targeting macrophages / |