Screen LibraryRNA nanostructures : methods and protocols /
This volume presents a collection of computational and experimental protocols pertaining to the creation, characterization, and utilization of RNA nanostructures. The chapters in this book cover topics such as ion effects in RNA folding; design and crystallography of self-assembling RNA nanostructur...
Saved in:
| Group Author: | ; |
|---|---|
| Published: |
Humana Press,
|
| Publisher Address: | New York, NY : |
| Publication Dates: | [2017] |
| Literature type: | Book |
| Language: | English |
| Series: |
Methods in molecular biology,
1632 Springer protocols |
| Subjects: | |
| Summary: |
This volume presents a collection of computational and experimental protocols pertaining to the creation, characterization, and utilization of RNA nanostructures. The chapters in this book cover topics such as ion effects in RNA folding; design and crystallography of self-assembling RNA nanostructures; x-aptamer selection and validation; RNAi in HIV-infected cells; and preparation of a conditional RNA switch. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, RNA Nanostructures: Methods and Protocols is a valuable resource for the design and production of RNA nanostructures. Researchers and scientists sharing these detailed protocols is important for sustained progress in the field. |
| Carrier Form: | xv, 383 pages : illustrations (some color) ; 27 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781493971374 1493971379 |
| Index Number: | QP623 |
| CLC: | Q522 |
| Call Number: | Q522/R486-23 |
| Contents: |
New method to predict ion effects in RNA folding / Computational generation of RNA nanorings / Protocols for molecular dynamics simulations of RNA nanostructures / Rolling circle transcription for the self-assembly of multimeric RNAi structures and its applications in nanomedicine / Computational prediction of the immunomodulatory potential of RNA sequences / Cotranscriptional production of chemically modified RNA nanoparticles / Supported fluid lipid bilayer as a scaffold to direct assembly of RNA nanostructures / Evaluation of thermal stability of RNA nanoparticles by temperature gradient gel electrophoresis (TGGE) in native condition / Design and crystallography of self-assembling RNA nanostructures / X-aptamer selection and validation / Design and preparation of aptamer-siRNA chimeras (AsiCs) for targeted cancer therapy / Cellular delivery of siRNAs using bolaamphiphiles / Preparation and optimization of lipid-like nanoparticles for mRNA Delivery / Chitosan nanoparticles for miRNA delivery / Synthesis of PLGA-lipid hybrid nanoparticles for siRNA delivery using the emulsion method PLGA-PEG-lipid nanoparticles for siRNA delivery / Oxime ether lipids as transfection agents : assembly and complexation with siRNA / Polycationic probe-guided nanopore single-molecule counter for selective miRNA detection / Intracellular reassociation of RNA-DNA hybrids that activates RNAi in HIV-infected cells / Construction and in vivo testing of prokaryotic riboregulators / Preparation of a conditional RNA switch / Rational engineering of a modular group I ribozyme to control its activity by self-dimerization / CRISPR-Cas RNA scaffolds for transcriptional programming in yeast / Using planar Phi29 pRNA three-way junction to control size and shape of RNA nanoparticles for biodistribution profiling in mice / |