Screen LibraryImaging gene expression : methods and protocols /
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| Group Author: | |
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| Published: |
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| Literature type: | Book |
| Language: | English |
| Series: |
Methods in molecular biology,
1042 Springer protocols |
| Subjects: | |
| Carrier Form: | xiii, 368 p. : ill. (some col.) cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781627035255 (alk. paper) : 1627035257 (alk. paper) |
| CLC: | Q786 |
| Call Number: | Q786/I318 |
| Contents: |
High-throughput fluorescence-based screen to identify factors involved in nuclear receptor recruitment to response elements / Live-cell imaging combined with immunofluorescence, RNA, or DNA FISH to study the nuclear dynamics and expression of the X-inactivation center / Single-molecule resolution fluorescent in situ hybridization (smFISH) in the yeast S. cerevisiae / Measuring transcription dynamics in living cells using fluctuation analysis / Tracking nuclear Poly(A) RNA movement within and among speckle nuclear bodies and the surrounding nucleoplasm / Nuclear trafficking and export of single, native mRNPs in Chironomus tentans salivary gland cells / Single mRNP tracking in living mammalian cells / Imaging nascent RNA dynamics in dictyostelium / Monitoring dynamic binding of chromatin proteins in vivo by single-molecule tracking / Single-particle tracking for studying the dynamic properties of genomic regions in live cells / Microscopic analysis of chromatin localization and dynamics in C. elegans / Measuring the dynamics of chromatin proteins during differentiation / Electron spectroscopic tomography of specific chromatin domains / BAC manipulations for making BAC transgene arrays / Spatiotemporal visualization of DNA replication dynamics / Dynamics of DNA damage repair and transcription / Fluorescence microscopy-based high-throughput screening for factors involved in gene silencing / Actin as a model for the study of nucleocytoplasmic shuttling and nuclear dynamics / Isochronal visualization of transcription and proteasomal proteolysis in cell culture or in the model organism, Caenorhabditis elegans / Considering discrete protein pools when measuring the dynamics of nuclear membrane proteins / Correlative microscopy of individual cells : sequential application of microscopic systems with increasing resolution to study the nuclear landscape / Time-lapse, photoactivation, and photobleaching imaging of nucleolar assembly after mitosis / Nucleation of nuclear bodies / |