Screen LibrarySeparation methods in organic chemistry and biochemistry /
Separation Methods in Organic Chemistry and Biochemistry aims to provide perspectives for the commonly used separations methods and to discuss indications for their use. The book discusses the determination of molecular properties useful in separation based on micro test methods, paper chromatograph...
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| Main Authors: | |
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| Corporate Authors: | |
| Published: |
Academic Press,
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| Publisher Address: | New York : |
| Publication Dates: | 1969. |
| Literature type: | eBook |
| Language: | English |
| Subjects: | |
| Online Access: |
http://www.sciencedirect.com/science/book/9780127616506 |
| Summary: |
Separation Methods in Organic Chemistry and Biochemistry aims to provide perspectives for the commonly used separations methods and to discuss indications for their use. The book discusses the determination of molecular properties useful in separation based on micro test methods, paper chromatography, thin-layer chromatography, and electrophoresis. The text then describes the theoretical principles of group-separation procedures, liquid-liquid partition, ion-exchange selectivity, gel permeation, and adsorption. Methods of influencing the selectivity coefficients, the basic theory of fractionat. |
| Carrier Form: | 1 online resource (viii, 237 pages) : illustrations |
| Bibliography: | Includes bibliographical references. |
| ISBN: |
9781483220680 1483220680 |
| Index Number: | QD63 |
| CLC: | O658 |
| Contents: |
Front Cover; Separation Methods in Organic Chemistry and Biochemistry; Copyright Page; Preface; Table of Contents; Introduction; I. TYPES OF SEPARATION CONSIDERED; II. DISTRIBUTION COEFFICIENT; A. Group Separation; B. Fractionation Separations-Reasons for Using Them; C. Methods of Fractionation; III. USE OF MICRO METHODS; IV. EVALUATION OF SEPARATION PROCESSES; Chapter I. General Principles; I. STABILITY; II. GROUP SEPARATIONS; A. Molecular Size; 1. Dialysis and Ultrafiltration; 2. Gel Filtration; B. Ionic Properties; 1. Ion Exchange; 2. Solvent Extraction; C. Polarity. 1. Definition and General Principles2. Solvent Extraction; D. Summation of Group Properties and Methods; III. FRACTIONATION SEPARATIONS; A. Requirements and General Methods; B. Countercurrent Distribution; 1. Procedure; 2. Parameters of the Separation Process-Relationship of Chromatography and Countercurrent Distribution; 3. Mathematical Description-Formation and Location of Bands; 4. Mathematical Description-Band Spread and Separation; chapter II. Determination of Molecular Properties; I. INTRODUCTION; II. CHROMATOGRAPHIC PROCEDURES; A. Visualization; 1. Direct Visualization. Chapter Ill. Solvent ExtractionI. PARTITION RATIO; A. Definition; B. Chemical Potential; 1. Activity Coefficient; 2. Relationship between Different Solvent Systems; I. Partition Ratio; C. Methods of Determining the Partition Ratio; 1. Paper Chromatography; 2. Equilibration between Two Phases; 3. Other Procedures; II. APPLICATION OF SOLVENT EXTRACTION FOR GROUP SEPARATION; A. Solvent Properties; 1. Partition Ratio; 2. Solvent Polarity; 3. Solvent Removal and Loss; 4. Reactivity; 5. Ease of Separation; B. Solvent Removal Methods; 1. Evaporation; 2. Back Extraction; 3. Adsorption. 4. PrecipitationIII. APPLICATION OF SOLVENT EXTRACTION FOR FRACTIONATION SEPARATION; IV. PARTITION CHROMATOGRAPHY; A. Properties of the Solid Support; B. Properties of the Solvent System; 1. Stability; 2. Partition Ratio; 3. Factors Influencing Column Load; C. Mechanical Problems; D. Mechanism of Separation; 1. Factors Influencing Plate Height in Columns; E. Applications of Partition Chromatography; 1. Insulin; 2. Aldosterone; 3. Use of Hydrophilic Gels; 4. Miscellaneous Applications; V. COUNTERCURRENT DISTRIBUTION; A. Physical and Chemical Requirements; 1 . Solvent System. |