Screen LibraryImmunochemical protocols
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| Group Author: | |
|---|---|
| Published: |
Humana Press,
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| Publisher Address: | Totowa, N.J. |
| Publication Dates: | c2005. |
| Literature type: | Book |
| Language: | English |
| Edition: | 3rd ed. |
| Series: |
Methods in molecular biology ; 295 |
| Subjects: | |
| Carrier Form: | viii, 317 p.: ill. ; 24 cm. |
| ISBN: |
1588292746 (alk. paper) 1592598730 (e-ISBN) |
| Index Number: | R392 |
| CLC: | R392.11-3 |
| Call Number: | R392.11-3/I33/3rd.ed. |
| Contents: |
Includes bibliographical references and index. Immunization strategies for antibody production / Robert Burns -- Synthetic peptides as antigens for antibody production / David C. Hancock and Nicola J. O'Reilly -- Production of polyclonal antibody in rabbits / David C. Hancock and Nicola J. O'Reilly -- Making hybridomas / Robert Burns -- Growing hybridomas / Gary Entrican and Gareth Young -- Phage display vectors for the in vitro generation of human antibody fragments / Michael Hust and Stefan Dobel -- Purification of antibodies and preparation of antibodies fragments / Graham D. Kemp -- Conjugation of antibodies to alkaline phosphatase / G. Brian Wisdom -- Conjugation of antibodies to horseradish peroxidase / G. Brian Wisdom -- Conjugation of antibodies to fluorescein or rhodamine / G. Brian Wisdom -- Conjugation of antibodies to biotin / James D. Hirsch and Rosaria P. Haughland -- Preparation of colloidal gold probes / David Hughes -- Immunogold probes in light microscopy / David Hughes -- Immunoelectron microscope techniques in plant virus diagnosis / Daphne M. Wright -- Dual-antibody sandwich enzyme-linked immunosorbent assay / William Jordan -- Competitive enzyme-linked immunosorbent assay / William Jordan -- Immunoblotting techniques / Barbara Magi and Sabrina Liberatori -- Epitope mapping / Glenn E. Morris -- Generation and testing of immunoaffinity columns / Weilin L. Shelver -- Immunocapture-polymerase chain reaction / Vincent Mulholland -- Magnetic cell sorting / Maciej Zborowski and Jeffrey J. Chalmers -- Cell enrichment and immunochemical staining / Oscar R. Lara and Jeffrey J. Chalmers. |