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Molecular imaging : FRET microscopy and spectroscopy /

The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and t...

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Bibliographic Details
Corporate Authors: Elsevier Science & Technology; American Physiological Society 1887
Group Author: Periasamy, Ammasi; Day, Richard N
Published: Published for the American Physiological Society by Oxford University Press,
Publisher Address: Oxford ; New York :
Publication Dates: 2005.
Literature type: eBook
Language: English
Series: The American Physiological Society methods in physiology series
Subjects:
Fluorescence microscopy
Fluorescence spectroscopy
Microscopie de fluorescence
Spectroscopie de fluorescence
NATURE > Reference
SCIENCE > Life Sciences > General
SCIENCE > Life Sciences > Biology
Fluorescence Resonance Energy Transfer
Microscopy, Fluorescence
Microscopy, Fluorescence > methods
Molecular Imaging
Spectrometry, Fluorescence
Spectrometry, Fluorescence > methods
Electronic books
Online Access: http://www.sciencedirect.com/science/book/9780195177206
Summary: The detection and measurement of the dynamic interactions of proteins within the living cell are critical to our understanding of cell physiology and pathophysiology. With FRET microscopy and spectroscopy techniques, basic and clinical scientists can make such measurements at very high spatial and temporal resolution. But sources of background information about these tools are very limited, so this book fills an important gap. It covers both the basic concepts and theory behind the various FRET microscopy and spectroscopy techniques, and the practical aspects of using the techniques and anal
Carrier Form: 1 online resource (xv, 312 pages) : illustrations (some color).
Bibliography: Includes bibliographical references and index.
ISBN: 9780195177206
0195177207
9780080536873
0080536875
Index Number: QH212
CLC: Q-336
Contents: Proteins and the Flow of Information in Cellular Function; Basics of Fluorescence and FRET; An Introducrion to Filters and Mirrors for FRET; FRET Imaging in the Wide-field Microscope; Confocal FRET Microscopy -- Study of Clustered Distribution of Receptor-ligand Complexes in Endocytic Membranes; Multiphoton FRET Microscopy for Protein Localization in Tissue; Single-Molecule FRET; FRET Measurements using Multispectral Imaging; Real-time Fluorescence Lifetime Imaging and FRET using Fast Gated Image Intensifiers; Streak FLIM: A Novel Technology for Quantitative FRET Imaging; Time-Correlated Sin

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