Screen LibraryTechniques in protein chemistry VIII /
Praise for the Series: "The mainly sharp scientific focus of this set of snapshots is a credit to both the contricutors and the editorial team."--Biotechnology and Applied Biochemistry Techniques in Protein Chemistry VIII is the latest volume in this successful series. As a valuable bench-...
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| Corporate Authors: | ; |
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| Group Author: | |
| Published: |
Academic Press,
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| Publisher Address: | San Diego ; London : |
| Publication Dates: | 1997. |
| Literature type: | eBook |
| Language: | English |
| Subjects: | |
| Online Access: |
http://www.sciencedirect.com/science/bookseries/10808914/8 |
| Summary: |
Praise for the Series: "The mainly sharp scientific focus of this set of snapshots is a credit to both the contricutors and the editorial team."--Biotechnology and Applied Biochemistry Techniques in Protein Chemistry VIII is the latest volume in this successful series. As a valuable bench-top reference tool for protein chemists, the ten section sof the book are divided by subject area to show the reader which techniques are currently applied to particular problems in protein science. This approach reflects current trends in which specific instruments and methodologies are used in several different areas. * * The book features the latest advances in protein chemistry methodologies in the following areas: * Protein sequencing and amino acid analysis * Mass spectral analysis of peptides and proteins * Posttranslational processing * High-sensitivity protein and peptide separations * Protein folding and NMR * Functional domain analysis * Protein design and engineering * Three-dimensional protein structure. |
| Item Description: | "Selected from presentations at the Tenth Symposium of the Protein Society, held in San Jose, August 3-7, 1996"--Page xvii. |
| Carrier Form: | 1 online resource (xxi, 908 pages) : illustrations |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9780080542287 008054228X |
| Index Number: | QP551 |
| CLC: |
Q51 O629.73-53 |
| Contents: | Foreword. -- Preface. -- Acknowledgments. -- Primary Structural Analysis: Protein Sequencing Using Microreactors and Capillary Electrophoresis with Thermo-optical Absorbance Detection: -- Enhancement of Concentration Limits of Detection in Capillary Electrophoresis: Examples of On-Line Sample Preconcentration, Cleanup, and Microreactor Technology in Protein Characterization. -- Sequencing MHC Class I Peptides Using Membrane Preconcentration-Capillary Electrophoresis Tandem Mass Spectrometry (mPC-CE-MS/MS). -- Nano-electrospray Mass Spectrometry and Edman Sequencing of Peptides and Proteins Collected from Capillary Electrophoresis. -- Characterization of a Recombinant Hepatitis E Protein Vaccine Candidate by Mass Spectrometry and Sequencing Techniques. -- Comparison of the High Sensitivity and Standard Versions of Applied Biosystems Procise <V>TM</V> 494 N-Terminal Protein Sequencers Using Various Sequencing Supports. -- Evaluation of ABRF-96SEQ: A Sequence Assignment Exercise. -- Internal Protein Sequencing of SDS-PAGE-Separated Proteins: Optimization of an In Gel Digest Protocol. -- A Strategy to Obtain Internal Sequence Information from Blotted Proteins after Initial N-terminal Sequencing. -- Internal Protein Sequencing of SDS PAGE-Separated Proteins: A Collaborative ABRF Study. -- Physical and Chemical Analysis: -- Chromatographic Determination of Extinction Coefficients of Non-Glycosylated Proteins Using Refractive Index (RI) and UV Absorbance (UV) Detectors: Applications for Studying Protein Interactions by Size Exclusion Chromatography with Light-Scattering, UV, and RI Detectors. -- Single Alkaline Phosphatase Molecule Assay by Capillary Electrophoresis Laser-Induced Fluorescence Detection. -- A New Centrifugal Device Used in Sample Clean-up and Concentration of Peptides. -- Sample Preparation Using Synthetic Membrances for the Study of Biopolymers by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry. -- Use of LC/MS Peptide Mapping for Characteriation of Isoforms in <V>15</V>N-Labeled Recombinant Human Leptin. -- Hyphenated HPLC Methodology for the Resolution and Elucidation of Peptides from Proteolytic Digests. -- Detecting and Identifying Active Compounds from a Combinatorial Library Using Iasys and Electrospray Mass Spectrometry. -- Amino Acid Analysis of Unusual and Complex Samples Based on 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate Derivatization. -- Development of a Method for Analysis of Free Amino Acids from Physiological Samples Using a 420A ABI/PE Amino Acid Analyzer. -- Quantitation and Identification of Proteins by Amino Acid Analysis: ABRF-96AAA Collaborative Trial -- Chemical Modification: -- Nonaqueous Chemical Modification of Lyophilized Proteins -- Reaction of HIV-1 NC p7 Zinc Fingers with Electrophilic Reagents. -- The Identification and Isolation of Reactive Thiols in Ricin A-Chain and Blocked Ricin Using 2-(4'-Maleimidylanilino)naphthalene-6-sulfonic Acid. -- Inactivation of the Human Cytomegalovirus Protease by Diisopropylfluorophosphate. -- Studies on the Status of Arginine Residues in Phospholipase A<->2 from Naja naja atra (Taiwan cobra) Snake Venom. -- Selective Reduction of the Intermolecular Disulfide Bridge in Human Glial Cell Lined-Derived Neurotrophic Factor Using Tris-(2-Carboxyethyl)Phosphine. -- Effects of Surface Hydrophobicity on the Structural Properties of Insulin. -- The Effects of in Vitro Methionine Oxidation on the Bioactivity and Structure of Human Keratinocyte Growth Factor. -- Posttranslational and Other Modifications: -- Effects of Enzyme Glycosylation on the Chemical Step of Catalysis, as Probed by Hydrogen Tunneling and Enthalpy of Activation. -- Profile Analysis of Oligosaccharides from Glycoproteins by PMP Labeling. -- Comparison of Chemical and Enzymatic Release Methods Using RP-HPLC and Mass Spectrometry. -- Positive Identification of Glycosylation Sites in Proteins and Peptides Using a Modified Beckman LF 3600 N-Terminal Protein Sequencer. -- Deamidation and Isoaspartate Formation during in Vitro Aging of a Recombinant Hepatitis E Vaccine Candidate. -- The Isolation and Characterization of Active Site Peptides in Lysyl Oxidase. -- Complement Activation in EDTA Blood/Plasma Samples May Be Caused by Coagulation Proteases. -- Disulfide-Linked Human Stem Cell Factor Dimer: Method of Identification and Molecular Comparison to the Noncovalent Dimer. -- Autocatalytic Reduction of a Humanized Antibody. -- Interactions of Protein with Ligands: -- Oxygen and Ascorbate Mediated Modification of a Recombinant Hemoglobin. -- Metal Activation and Regulation of E. coli Rnase H. Crystal Structure of Avian Sarcoma Virus Integrase with Bound Essential Cations. -- Multidimensional NMR Studies of an Exchangeable Apolipoprotein and Its Interactions with Lipids. -- NMR Methods for Analysis of CRALBP Retinoid Binding. -- Novel Method for Measuring the Binding Properties of the Site-Directed Mutants of the Proteins that Bind Hydrophobic Ligands: Applicati |