Screen LibraryProteomics in practice：a guide to successful experimental design
Saved in:
| Main Authors: | |
|---|---|
| Group Author: | ; |
| Published: |
Wiley-VCH,
|
| Publisher Address: | Weinheim |
| Publication Dates: | 2008. |
| Literature type: | Book |
| Language: | English |
| Edition: | 2nd ed. |
| Subjects: | |
| Carrier Form: | xx, 482 p.: ill. (some col.) ; 25 cm. |
| ISBN: |
9783527319411 (cased) 3527319417 (cased) |
| Index Number: | Q510 |
| CLC: | Q510.3-62 |
| Call Number: | Q510.3-62/W527/2nd.ed. |
| Contents: |
Previous ed.: 2002. Includes bibliographical references and index. Preface -- Foreword -- Abbreviations, symbols, units -- Introduction -- Pt. I. Proteomics technology. Electrophoretic techniques ; Liquid chromatography techniques ; Mass spectrometry ; Functional proteomics: studies of protein-protein interactions -- Pt. II. Practical manual of proteome analysis. Equipment, consumables, reagents ; Step 1: Sample preparation ; Step 2: Fluorescence different gel electrophoresis ; Step 3: Isoelectric focusing ; Step 4: SDS polyacrylamide gel electrophoresis ; Step 5: Scanning of gels containing pre-labeled proteins ; Step 6: Staining of gels ; Step 7: Image analysis and evaluation of DIGE gels ; Step 8: Spot excision ; Step 9: Sample destaining ; Step 10: Protein digestion ; Step 11: Microscale desalting and concentrating of sample ; Step 12: Chemical derivatization of the peptide digest ; Step 13: MS analysis ; Step 14: Calibration of MALDI-ToF MS ; Step 15: Preparing for a database search -- Pt. III. Trouble shooting -- Glossary of terms. |