Screen LibraryGTPases regulating membrane dynamics
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| Group Author: | ; ; |
|---|---|
| Published: |
Academic Press,
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| Publisher Address: | San Diego, Calif. |
| Publication Dates: | c2005. |
| Literature type: | Book |
| Language: | English |
| Series: |
Methods in enzymology ; 404 |
| Subjects: | |
| Carrier Form: | xliii, 692 p.: ill. (some col.) ; 24 cm. |
| ISBN: |
9780121828097 0121828093 |
| Index Number: | Q525 |
| CLC: |
Q525 Q55 |
| Call Number: | Q55/G895 |
| Contents: |
Includes bibliographical references and indexes. Arrays of transfected mammalian cells for high content screening microscopy -- Imaging platforms for measurement of membrane trafficking -- Bioinformatic "harvester": A search engine for genome-wide human, mouse and rat protein resources -- Microinjection as a tool to explore small GTPase function -- Visualizing intracellular events in vivo by combined video fluorescence and 3-D electron microscopy -- Recombinant production in baculovirus-infected insect cells and purification of the mammalian sec13-sec31 complex -- Purification and properties of mammalian sec-23/24 from insect cells -- Purification and functional properties of yeast sec12 GEF -- Reconstitution of cargo-dependent COPII coat assembly on proteoliposomes -- Real-time assays for the assembly-disassembly cycle of COP coats on liposomes of defined size -- ... |