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Culture of animal cells : a manual of basic technique and specialized applications /

Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made - particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Sev...

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Bibliographic Details
Main Authors: Freshney, R. Ian.
Corporate Authors: Wiley (Online service)
Group Author: Capes-Davis, Amanda; Gregory, Carl; Przyborski, Stefan
Published: Wiley-Blackwell,
Publisher Address: Hoboken, New Jersy :
Publication Dates: [2016]
Literature type: eBook
Language: English
Edition: Seventh edition.
Subjects:
Tissue culture > Laboratory manuals.
Cell culture > Laboratory manuals.
Cell culture.
Tissue culture.
Cell Culture Techniques.
Tissue Culture Techniques.
Online Access: http://www.itextbook.cn/f/book/bookDetail?bookId=87d9bf855d544ea0b415e024ab0b0d16
Summary: Since the publication of the sixth edition of this benchmark text, numerous advances in the field have been made - particularly in stem cells, 3D culture, scale-up, STR profiling, and culture of specialized cells. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition is the updated version of this benchmark text, addressing these recent developments in the field as well as the basic skills and protocols. This eagerly awaited edition reviews the increasing diversity of the applications of cell culture and the proliferation of specialized techniques, and provides an introduction to new subtopics in mini-reviews. New features also include a new chapter on cell line authentication with a review of the major issues and appropriate protocols including DNA profiling and barcoding, as well as some new specialized protocols. Because of the continuing expansion of cell culture, and to keep the bulk of the book to a reasonable size, some specialized protocols are presented as supplementary material online. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.
Item Description: Description based on print version record.
Carrier Form: 1 online resource (xxxviii, 684 pages, [32] unnumbered pages of plates) : illustrations (some color)
Bibliography: Includes bibliographical references and index.
ISBN: 9781118873373
Index Number: QH585
CLC: Q954.6-33
Contents: Introduction -- Biology of Cultured Cells -- Laboratory Design and Layout -- Equipment and Materials -- Aseptic Technique -- Safety, Bioethics, and Validation -- Culture Vessels and Substrates -- Defined Media and Supplements -- Serum-Free Media -- Preparation and Sterilization ; Supplementary Material -- Primary Culture ; Supplementary Material -- Subculture and Cell Lines -- Authentication and Validation -- Microbial Contamination -- Cryopreservation and banking -- Cloning and Selection ; Supplementary Material -- Cell Sorting -- Cell Line Characterization -- Differentiation -- Three-Dimensional Culture -- Transformation and Immortalization -- Scale-up and Automation -- Senescence, Immortalization, and Transformation -- Quantitation ; Supplementary Material -- Cytotoxicity ; Supplementary Material -- Culture of Specific Cell Types ; Supplementary Material -- Stem Cells ; Supplementary Material -- Training Programs -- Problem Solving -- In Conclusion ; Supplementary Material.

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