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Author: 金皮迈基,埃西

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Human respiratory syncytial virus : methods and protocols /

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Bibliographic Details
Group Author: Tripp, Ralph A. (Editor); Jorquera, Patricia A. (Editor)
Published: Humana Press,
Publisher Address: New York, NY :
Publication Dates: [2016]
Literature type: Book
Language: English
Series: Springer protocols
Methods in molecular biology, 1442
Subjects:
Respiratory syncytial virus > Laboratory manuals.
Carrier Form: xi, 247 pages : illustrations (some color) ; 27 cm.
Bibliography: Includes bibliographical references and index.
ISBN: 9781493936854
1493936859
Index Number: RC740
CLC: R373.1-33
Call Number: R373.1-33/H918
Contents: Human respiratory syncytial virus : an introduction /
RSV growth and quantification by microtitration and qRT- PCR assays /
Quantification of RSV infectious particles by plaque assay and immunostaining assay /
Detection of RSV antibodies in human plasma by enzyme immunoassays /
Secretory expression and purification of respiratory syncytial virus G and F proteins in human cells /
Development of human monoclonal antibodies against respiratory syncytial virus using a high efficiency human hybridoma technique /
Respiratory syncytial virus (RSV) : neutralizing antibody, a correlate of immune protection /
Host factors modulating RSV infection : use of small interfering RNAs to probe functional importance /
In vitro modeling of RSV infection and cytopathogenesis in well-differentiated human primary airway epithelial cells (WD-PAECs) /
Reverse genetics of respiratory syncytial virus /
Use of minigenome systems to study RSV transcription /
Screening for host factors directly interacting with RSV protein : microfluidics /
Proteomic-based workflow using purified respiratory syncytial virus particles to identify cellular factors as drug targets /
MicroRNA profiling from RSV-infected biofluids, whole blood, and tissue samples /
Mouse and cotton rat models of human respiratory syncytial virus /
In vivo assessment of airway function in the mouse model /
Evaluation of the adaptive immune response to respiratory syncytial virus /

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