Screen LibraryFunctional genomics : methods and protocols /
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| Group Author: | ; ; |
|---|---|
| Published: |
Humana Press : Springer,
|
| Publisher Address: | New York, NY : |
| Publication Dates: | [2017] |
| Literature type: | Book |
| Language: | English |
| Edition: | Third edition. |
| Series: |
Methods in molecular biology,
1654 Springer protocols |
| Subjects: | |
| Carrier Form: | xv, 392 pages : illustrations (some color) ; 26 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781493972302 1493972308 |
| Index Number: | QH447 |
| CLC: | Q523 |
| Call Number: | Q523/F979/3rd ed. |
| Contents: |
Predicting RNA structure with Vfold / RNA function prediction / Computational prediction of novel miRNAs from genome-wide data / Protein structure modeling with MODELLER / Protein function prediction / Capturing three-dimensional genome organization in individual cells by single-cell hi-c / Genome-wide cell type-specific mapping of in vivo chromatin protein binding using an FLP-inducible DamID system in Drosophila / DNA methylation profiling using long-read single molecule real-time bisulfite sequencing (SMRT-BS) / Copy number variation analysis by droplet digital PCR / MicroScale thermophoresis : a rapid and precise method to quantify protein-nucleic acid interactions in solution / Establishment of the CRISPR/Cas9 system for targeted gene disruption and gene tagging / Holistic and affordable analyses of MicroRNA expression profiles using tagged cDNA libraries and a multiplex sequencing strategy / MicroRNA expression analysis using small RNA sequencing discovery and RT-qPCR-based validation / Using FirePlex particle technology for multiplex microRNA profiling without RNA purification / Multiplex real-time PCR using encoded microparticles for microRNA profiling / Optimized whole transcriptome profiling of motor axons / 2D-DIGE in proteomics / STAGE-diging in proteomics / Protein arrays I : antibody arrays / Protein arrays II : antigen arrays / Protein arrays III : reverse-phase protein arrays / Isolation of exosomes for the purpose of protein cargo analysis with the use of mass spectrometry / Virus-induced gene silencing (VIGS) and foreign gene expression in Pisum sativum L. Using the "One-Step" Bean pod mottle virus (BPMV) viral vector / Re-expressing epigenetically silenced genes by inducing DNA demethylation through targeting of ten-eleven translocation 2 to any given genomic locus / Knockdown of rice microRNA166 by short tandem target mimic (STTM) / RNAi-mediated knockdown of protein expression / Engineered zinc finger DNA-binding domains : synthesis, assessment of DNA-binding affinity, and direct protein delivery to mammalian cells / Production, purification, and titration of first-generation adenovirus vectors / |