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G proteins /

This volume presents biochemical and molecular techniques for the functional characterization of G proteins. Detailed methodologies are described for the production and purification of recombinant G protein subunits, for the manipulation of both sense and antisense G proteins RNA and DNA.

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Bibliographic Details
Corporate Authors: Elsevier Science & Technology.
Group Author: Roche, Patrick C. (Editor)
Published: Academic Press,
Publisher Address: San Diego :
Publication Dates: 1996.
Literature type: eBook
Language: English
Series: Methods in neurosciences, v. 29
Subjects:
G proteins.
GTP-Binding Proteins > physiology.
SCIENCE > Biochemistry. > Biochemistry.
Electronic books.
Online Access: http://www.sciencedirect.com/science/bookseries/10439471/29
Summary: This volume presents biochemical and molecular techniques for the functional characterization of G proteins. Detailed methodologies are described for the production and purification of recombinant G protein subunits, for the manipulation of both sense and antisense G proteins RNA and DNA.
Carrier Form: 1 online resource (xviii, 409 pages) : illustrations.
Bibliography: Includes bibliographical references and index.
ISBN: 9780121852993
0121852997
9780080532271
0080532276
1281059285
9781281059284
Index Number: QP552
CLC: Q51
Contents: G proteins controlling differentiation, growth, and development : analysis by antisense RNA/DNA technology / Christopher M. Moxham, Hsien-yu Wang, Craig C. Malbon -- Analysis of adenylylcyclase subspecies gene expression in brain by in situ hybridization histochemistry / Nicole Mons -- Site-directed mutagenesis of G protein [alpha] subunits : analysis of [alpha] phosphorylation and fatty acid acylation / Hazem Hallak, Karen M. Lounsbury, and David R. Manning -- G protein activity in solubilized membrane preparations / Irene Litosch and Hong Xie -- Cross-linking of synaptoneurosome G proteins / Sherry Coulter, Saleem Jahangeer, and Martin Rodbell -- Quantitative reconstitution and ADP-ribosylation assays for G proteins / Joel Abramowitz -- Functional assays to study coupling of dopamine D2 receptors to G proteins / Ja-Hyun Baik [and others] -- Assays for G protein [beta]y subunit activity / Joyce B. Higgins and Patrick J. Casey -- [alpha] -adrenergic receptor coupling to G proteins / Ann E. Remmers -- Somatostatin receptor coupling to G proteins / John R. Hadcock and Joann Strnad -- Galanin receptor-G protein interactions and stimulation of GTPase activity / Susan L. Gillison, Gunnar Skoglund, and Geoffrey W.G. Sharp -- Cloning and site-directed mutagenesis studies of gonadotropin-releasing hormone receptor / Stuart C. Sealfon [and others] -- Use of the Xenopus laevis expression system for structure-function analysis of G-protein-coupled receptor for parathyroid hormone and parathyroid hormone-related protein / Paul R. Turner [and others] -- Production of G protein [alpha]-subunit-specific monoclonal antibodies / Xiaohua Li and Richard S. Jope -- Expression and purification of G protein [beta]y subunits using baculovirus expression system / Stephen G. Graber, Margaret A Lindorfer, and James C. Garrison -- Analysis of G protein [gamma] subunits using baculovirus expression system : requirement for posttranslational processing / Janet D. Robishaw -- Expression, purification, and functional reconstitution of recombinant phospholipase C-[beta] isozymes / Andrew Paterson and T. Kendall Harden -- Measuring cooperative binding between retinal rod G protein, transducin, and activated rhodospin / Barry M. Willardson, Tatsuro Yoshida, and Mark W. Bitensky -- Crosstalk between tyrosine kinase and G-protein-linked signal transduction systems / Ross D. Feldman and Michel Bouvier -- Subcellular distribution and posttranslational modifications of GTP-binding proteins in insulin-secreting cells / Anjaneyulu Kowluru and Stewart A. Metz -- Epidermal growth factor-mediated regulation of G proteins and adenylylcylase in cardiac muscle / Tarun B. Patel [and others] -- G protein dependence of [alpha] -adrenergic receptor subtype action in cardiac myocytes / Vitalyi Rybin, Hyung-Mee Han, and Susan F. Steinberg -- G[subscript s][alpha] mutations and pituitary adenomas / Joseph M. Alexander -- Molecular methods for analysis of genetic polymorphisms : application to the molecular genetic study of genes encoding [beta] -adrenoceptor and stimulatory G protein [alpha] subunit / Charles W. Emala and Michael A. Levine.

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