Screen LibrarySelected papers of Frederick Sanger : with commentaries /
This important volume is mainly concerned with the development of methods for "sequencing" - that is, determination of the order of the amino acids in proteins and of nucleotides in RNA and DNA. In 1943 the position of only one amino acid in a protein (insulin) was known, and Sanger's...
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| Corporate Authors: | |
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| Group Author: | ; |
| Published: |
World Scientific Pub. Co.,
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| Publisher Address: | Singapore : |
| Publication Dates: | 1996. |
| Literature type: | eBook |
| Language: | English |
| Series: |
World Scientific series in 20th century biology ;
vol. 1 |
| Subjects: | |
| Online Access: |
http://www.worldscientific.com/worldscibooks/10.1142/2902#t=toc |
| Summary: |
This important volume is mainly concerned with the development of methods for "sequencing" - that is, determination of the order of the amino acids in proteins and of nucleotides in RNA and DNA. In 1943 the position of only one amino acid in a protein (insulin) was known, and Sanger's first paper resulted in finding a second amino acid. In his final paper in 1982 he describes the determination of a DNA sequence of 48,502 nucleotides. The papers describe the steady improvements in techniques, and exciting biological results revealed by the sequences. |
| Carrier Form: | 1 online resource (xiii,662pages) : illustrations. |
| Bibliography: | Includes bibliographical references. |
| ISBN: | 9789812830630 |
| Index Number: | QP551 |
| CLC: | Q51 |
| Contents: |
pt. I. Proteins. Comments on papers 1 to 19. 1. The free amino groups of insulin -- 2. Fractionation of oxidized insulin -- 3. The terminal peptides of insulin -- 4. The amino acid sequence in the phenylalanyl chain of insulin 1. The identification of lower peptides from partial hydrolysates (with H. Tuppy) -- 5. The amino acid sequence in the phenylalanyl chain of insulin. 2. The investigation of peptides from enzymic hydrolysates (with H. Tuppy) -- 6. The amino acid sequence in the glycyl chain of insulin. 1. The identification of lower peptides from partial hydrolysates (with E.O.P. Thomp 35. DNA sequence at the C termini of the overlapping genes A and B in bacteriophage [symbol]X174 (with M. Smith ... [et al.]) -- 36. Nucleotide sequence of bacteriophage [symbol]X174 DNA (with G.M. Air ... [et al.]) -- 37. DNA sequencing with chain-terminating inhibitors (with S. Nicklen and A.R. Coulson) -- 38. The use of thin acrylamide gels for DNA sequencing (with A.R. Coulson) -- 39. The nucleotide sequence of bacteriophage [symbol]X174 (with A.R. Coulson ... [et al.]) -- 40. Nucleotide sequence of the F protein coding region of bacteriophage [symbol]X174 and the amino acid sequence of |