Screen LibraryCRISPR-Cas : a laboratory manual /
Saved in:
| Group Author: | ; |
|---|---|
| Published: |
Cold Spring Harbor Laboratory Press,
|
| Publisher Address: | Cold Spring Harbor, New York : |
| Publication Dates: | [2016] |
| Literature type: | Book |
| Language: | English |
| Subjects: | |
| Carrier Form: | x, 181 pages : illustrations (some color) ; 29 cm |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781621821311 (paperback : alkaline paper) : 1621821315 (paperback : alkaline paper) 9781621821304 (hardcover : alkaline paper) 1621821307 (hardcover : alkaline paper) |
| Index Number: | QP623 |
| CLC: | Q522 |
| Call Number: | Q522/C932 |
| Contents: | 1. Overview of CRISPR- as9 Biology -- 2. Guide RNAs : A Glimpse at the Sequences that Drive CRISPR- as Systems -- 3. Characterization of Cas9- uide RNA Orthologs -- 4. Large-Scale Single Guide RNA Library Construction and Use for CRISPR- as9-Based Genetic Screens -- 5. Adeno-Associated Virus- ediated Delivery of CRISPR- as Systems for Genome Engineering in Mammalian Cells -- 6. Detecting Single-Nucleotide Substitutions Induced by Genome Editing -- 7. CRISPR- as9 Genome Engineering in Saccharomyces cerevisiae Cells -- 8. Cas9-Mediated Genome Engineering in Drosophila melanogaster -- 9. Optimization Strategies for the CRISPR- as9 Genome-Editing System -- 10. Editing the Mouse Genome Using the CRISPR- as9 System -- 11. Genome Editing in Human Pluripotent Stem Cells -- 12. An Introduction to CRISPR Technology for Genome Activation and Repression in Mammalian Cells. |