Screen LibraryDNA repair protocols /
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| Group Author: | |
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| Published: |
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| Literature type: | Book |
| Language: | English |
| Edition: | 3rd ed. |
| Series: |
Methods in molecular biology,
920 Springer protocols |
| Subjects: | |
| Carrier Form: | xvii, 631 p. : ill. (some col.) ; 27 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781617799976 (hbk. : alk. paper) : 1617799971 (hbk. : alk. paper) 9781617799983 (eBook) 161779998X (eBook) |
| Index Number: | QH467 |
| CLC: |
Q343-33 Q959.803-33 Q523-33 |
| Call Number: | Q523-33/D418-2/3rd ed. |
| Contents: |
DNA repair : front and center and not going away! / Identification of genes required for damage survival using a cell-based RNAi screen against the Drosophila genome / Isolating genes involved with genotoxic drug response in the nematode Caenorhabditis elegans using genome-wide RNAi screening / Establishment of the DNA repair-defective mutants in DT40 cells / Electrophoretic mobility shift assays for protein-DNA complexes involved in DNA repair / Comet assay : a sensitive genotoxicity test for the detection of DNA damage and repair / Detection of DNA damage by comet fluorescence in situ hybridization / Quantitative DNA damage and repair measurement with the yeast comet assay / Analysis of DNA damage and repair in nuclear and mitochondrial DNA of animal cells using quantitative PCR / In vitro DNA mismatch repair in human cells / Eukaryotic DNA mismatch repair in vitro / Immuno-slot blot assay for detection of UVR-mediated DNA damage / Quantification of DNA photoproducts in mammalian cell DNA using radioimmunoassay / Measuring the formation and repair of UV damage at the DNA sequence level by ligation-mediated PCR / Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies / Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts / In vitro base excision repair using mammalian cell extracts / Using Arabidopsis cell extracts to monitor repair of DNA base damage in vitro / In vitro monitoring of base excision repair in Saccharomyces cerevisiae / Mitochondrial base excision repair assays / O-hydroxylamine-coupled alkaline gel electrophoresis assay for the detection and measurement of DNA single-strand breaks / DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis / Quantitative amplification of single-stranded DNA / Simple, non-radioactive measurement of single-stranded DNA at telomeric, sub-telomeric, and genomic loci in budding yeast / Monitoring DNA recombination initiated by HO endonuclease / Monitoring I-SceI-induced double-strand break repair in DT40 Cells / I-SceI-based assays to examine distinct repair outcomes of mammalian chromosomal double strand breaks / Studying repair of a single protein-bound nick in vivo using the Flp-nick system / Measurement of diversification in the immunoglobulin light chain gene of DT40 cells / Live cell microscopy of DNA damage response in Saccharomyces cerevisiae / Use of gene targeting to study recombination in mammalian cell DNA repair mutants / In vitro rejoining of double strand breaks in genomic DNA / In vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides / Assays of bypass replication of genotoxic lesions in cell-free extracts / Quantitative measurement of translesion DNA synthesis in mammalian cells / Detection of PCNA modifications in Saccharomyces cerevisiae / In vitro PCNA modification assays / Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication / CHK1 kinase activity assay / Histone H2AX phosphorylation : a marker for DNA damage / |