Methods in enzymology. Volume 570, Chemokines /

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Bibliographic Details
Group Author: Handel, Tracy M. (Editor)
Published: Elsevier Academic Press,
Publisher Address: Cambridge, MA :
Publication Dates: 2016.
Literature type: Book
Language: English
Edition: First edition.
Series: Methods in enzymology, volume 570
Subjects:
Carrier Form: xxii, 632 pages, 20 unnumbered pages of plates : illustrations (some color) ; 24 cm.
Bibliography: Includes bibliographical references and indexes.
ISBN: 9780128021712
0128021713
Index Number: QR185
CLC: Q55-3
Call Number: Q55-3/M592/v.570
Contents: Chemokine detection using recetors immobilized on an SPR sensor surface -- Study of chemotaxis and cell-cell interactions in cancer with microfluidic devices -- Generating chemokine analogs with enhanced pharmacological properties using phage display -- Methods for the recognition of GAG-bound chemokines -- Monitoring scavenging activity of chemokine receptors -- Dual-color luciferase complementation for chemokine receptor signaling -- Analysis of arrestin recruitment to chemokine receptors by bioluminescence resonance energy transfer -- Probing biased signaling in chemokine receptors -- Mutagenesis by phage display -- Studying chemokine control of neutrophil migration in vivo in a murine model of inflammatory arthritis -- Production of chemokine/chemokine receptor complexes for structural biophysical studies -- In vivo models to study chemokine biology -- Monitoring chemokine receptor trafficking by confocal immunofluorescence microscopy -- Active shaping of chemokine gradients by atypical chemokine receptors: A 4D live-cell imaging migration assay -- The role of chemokine and glysocaminoglycan interaction in chemokine-mediated migration in vitro and in vivo -- Examining roles of glycans in chemokine-mediated dendritic-endothelial cell interactions -- Preparation and analysis of N-terminal chemokine receptor sulfopeptides using tyrosylprotein sulfotransferase enzymes -- Disulfide trapping for modeling and structure determination of receptor:chemokine complexes -- Analysis of G protein and [beta]-arrestin activation in chemokine receptors signaling -- Flow cytometry detection of chemokine receptors for the indentification of murine monoctye and neutrophil subsets -- Molecular pharmacology of chemokine receptors -- Preparation and characterization of glycosaminoglycan chemokine coreceptors -- Production of recombinant chemokines and validation of refolding -- Quantitative analysis of dendritic cell hapotaxis.