Methods in enzymology. Volume 570, Chemokines /
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Group Author: | |
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Published: |
Elsevier Academic Press,
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Publisher Address: | Cambridge, MA : |
Publication Dates: | 2016. |
Literature type: | Book |
Language: | English |
Edition: | First edition. |
Series: |
Methods in enzymology,
volume 570 |
Subjects: | |
Carrier Form: | xxii, 632 pages, 20 unnumbered pages of plates : illustrations (some color) ; 24 cm. |
Bibliography: | Includes bibliographical references and indexes. |
ISBN: |
9780128021712 0128021713 |
Index Number: | QR185 |
CLC: | Q55-3 |
Call Number: | Q55-3/M592/v.570 |
Contents: | Chemokine detection using recetors immobilized on an SPR sensor surface -- Study of chemotaxis and cell-cell interactions in cancer with microfluidic devices -- Generating chemokine analogs with enhanced pharmacological properties using phage display -- Methods for the recognition of GAG-bound chemokines -- Monitoring scavenging activity of chemokine receptors -- Dual-color luciferase complementation for chemokine receptor signaling -- Analysis of arrestin recruitment to chemokine receptors by bioluminescence resonance energy transfer -- Probing biased signaling in chemokine receptors -- Mutagenesis by phage display -- Studying chemokine control of neutrophil migration in vivo in a murine model of inflammatory arthritis -- Production of chemokine/chemokine receptor complexes for structural biophysical studies -- In vivo models to study chemokine biology -- Monitoring chemokine receptor trafficking by confocal immunofluorescence microscopy -- Active shaping of chemokine gradients by atypical chemokine receptors: A 4D live-cell imaging migration assay -- The role of chemokine and glysocaminoglycan interaction in chemokine-mediated migration in vitro and in vivo -- Examining roles of glycans in chemokine-mediated dendritic-endothelial cell interactions -- Preparation and analysis of N-terminal chemokine receptor sulfopeptides using tyrosylprotein sulfotransferase enzymes -- Disulfide trapping for modeling and structure determination of receptor:chemokine complexes -- Analysis of G protein and [beta]-arrestin activation in chemokine receptors signaling -- Flow cytometry detection of chemokine receptors for the indentification of murine monoctye and neutrophil subsets -- Molecular pharmacology of chemokine receptors -- Preparation and characterization of glycosaminoglycan chemokine coreceptors -- Production of recombinant chemokines and validation of refolding -- Quantitative analysis of dendritic cell hapotaxis. |