Screen LibraryMass spectrometry of glycoproteins : methods and protocols /
This comprehensive new resource in the 'Methods in Molecular Biology' series features contributions from leading researchers who provide expert advice and reproducible, cutting-edge protocols for examining glycoproteins through mass spectrometry.
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| Group Author: | ; |
|---|---|
| Published: |
Humana Press,
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| Publisher Address: | New York : |
| Publication Dates: | [2013] |
| Literature type: | Book |
| Language: | English |
| Series: |
Methods in molecular biology,
951 Springer Protocols |
| Subjects: | |
| Summary: |
This comprehensive new resource in the 'Methods in Molecular Biology' series features contributions from leading researchers who provide expert advice and reproducible, cutting-edge protocols for examining glycoproteins through mass spectrometry. |
| Carrier Form: |
xi, 339 pages : illustrations ; 26 cm. Also available in electronic format via the World Wide Web. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781627031455 (hardback : alkaline paper) : 1627031456 (hardback : alkaline paper) |
| Index Number: | QP552 |
| CLC: |
O657.63 Q513 |
| Call Number: | Q513/M414 |
| Contents: |
Introduction to glycosylation and mass spectrometry / PART I ENRICHMENT AND ISOLATION METHODS -- Tandem lectin weak affinity chromatography for glycoprotein enrichment / CSC technology: Selective labeling of glycoproteins by mild oxidation to phenotype cells / Use of boronic acid nanoparticles in glycoprotein enrichment / Incorporation of unnatural sugars for the identification of glycoproteins / Characterization of membrane-associated glycoproteins using lectin affinity chromatography and mass spectrometry / Sialic acid capture-and-release and LC-MS(n) analysis of glycopeptides / PART II SAMPLE PREPARATION -- In-solution digestion of glycoproteins for glycopeptide-based mass analysis / Nano-HPLC-MS of glycopeptides obtained after nonspecific proteolysis / PART III SEPARATION METHODS -- Glycopeptide enrichment for MALDI-TOF mass spectrometry analysis by hydrophilic interaction liquid chromatography solid phase extraction (HILIC SPE) / Separation and identification of glycoforms by capillary electrophoresis with electrospray ionization mass spectrometric detection / Structural separations by ion mobility-MS for glycomics and glycoproteomics / PART IV QUANTITATION METHODS -- Quantitative analysis of glycoprotein glycans / Stable isotope labeling of N-glycosylated peptides by enzymatic deglycosylation for mass spectrometry-based glycoproteomics / Approaches for site mapping and quantification of o-linked glycopeptides / Glycan profiling: Label-free analysis of glycoproteins / PART V COMPUTATIONAL TOOLS -- Introduction to informatics in glycoprotein analysis / Software tools for glycan profiling / PART VI CASE STUDIES IN MASS SPECTROMETRY OF GLYCOPROTEINS -- Quantitative characterization of glycoproteins in neurodegenerative disorders using iTRAQ / Quantitative proteomic analysis of N-linked glycoproteins in human tear fluid / Elucidation of N-glycosites within human plasma glycoproteins for cancer biomarker discovery / Characterizing the glycosylation state of therapeutic recombinant glycoproteins. / |