Culture of animal cells a manual of basic technique and specialized applications /

This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly...

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Bibliographic Details
Main Authors: Freshney, R. Ian
Corporate Authors: Wiley InterScience Online service
Published:
Literature type: Electronic eBook
Language: English
Edition: 6th ed.
Series: Wiley Online Library. Books
Subjects:
Online Access: http://onlinelibrary.wiley.com/book/10.1002/9780470649367
Summary: This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded sixth edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture.
Carrier Form: 1 online resource (xxxi, 732 p., [28] p. of plates) : ill. (some col.)
Bibliography: Includes bibliographical references (p. 663-715) and index.
ISBN: 9780470649367 (electronic bk.)
0470649364 (electronic bk.)
9780470528129
0470528125
9780470649350
0470649356
Index Number: QH585
CLC: Q813
Contents: Frontmatter -- Color Plates -- Introduction -- Biology of Cultured Cells -- Laboratory Design, Layout, and Equipment -- Equipment and Materials -- Aseptic Technique -- Safety, Bioethics, and Validation -- Culture Vessels and Substrates -- Defined Media and Supplements -- Serum-Free Media -- Preparation and Sterilization -- Primary Culture -- Subculture and Cell Lines -- Cloning and Selection -- Cell Separation -- Characterization -- Differentiation -- Transformation and Immortalization -- Contamination -- Cryopreservation -- Quantitation -- Cytotoxicity -- Specialized Cells -- Stem Cells, Ge