Culture of animal cells a manual of basic technique and specialized applications /
This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly...
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Main Authors: | |
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Published: |
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Literature type: | Electronic eBook |
Language: | English |
Edition: | 6th ed. |
Series: |
Wiley Online Library. Books |
Subjects: | |
Online Access: |
http://onlinelibrary.wiley.com/book/10.1002/9780470649367 |
Summary: |
This is the most complete resource on the techniques, equipment, principles, and practices of animal cell culture. Since publication of the previous edition of this benchmark text, numerous types of equipment and materials have changed and new specialized techniques have been developed, particularly in the area of stem cell research and tissue engineering. These and other developments have been incorporated into this rigorously revised and expanded sixth edition of Culture of Animal Cells, the leading text in the basic methodology of cell culture. |
Carrier Form: | 1 online resource (xxxi, 732 p., [28] p. of plates) : ill. (some col.) |
Bibliography: | Includes bibliographical references (p. 663-715) and index. |
ISBN: |
9780470649367 (electronic bk.) 0470649364 (electronic bk.) 9780470528129 0470528125 9780470649350 0470649356 |
Index Number: | QH585 |
CLC: | Q813 |
Contents: | Frontmatter -- Color Plates -- Introduction -- Biology of Cultured Cells -- Laboratory Design, Layout, and Equipment -- Equipment and Materials -- Aseptic Technique -- Safety, Bioethics, and Validation -- Culture Vessels and Substrates -- Defined Media and Supplements -- Serum-Free Media -- Preparation and Sterilization -- Primary Culture -- Subculture and Cell Lines -- Cloning and Selection -- Cell Separation -- Characterization -- Differentiation -- Transformation and Immortalization -- Contamination -- Cryopreservation -- Quantitation -- Cytotoxicity -- Specialized Cells -- Stem Cells, Ge |