Screen LibraryDirected evolution of selective enzymes : catalysts for organic chemistry and biotechnology /
Authored by one of the world's leading organic chemists, this authoritative reference provides an overview of basic strategies in directed evolution and introduces common gene mutagenesis, screening and selection methods. Throughout the text, emphasis is placed on methodology development to max...
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| Published: |
Wiley-VCH,
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| Publisher Address: | Weinheim, Germany : |
| Publication Dates: | 2016. |
| Literature type: | eBook |
| Language: | English |
| Subjects: | |
| Online Access: |
http://onlinelibrary.wiley.com/book/10.1002/9783527655465 |
| Summary: |
Authored by one of the world's leading organic chemists, this authoritative reference provides an overview of basic strategies in directed evolution and introduces common gene mutagenesis, screening and selection methods. Throughout the text, emphasis is placed on methodology development to maximize efficiency, reliability and speed of the experiments and to provide guidelines for efficient protein engineering. Professor Reetz highlights the application of directed evolution experiments to address limitations in the field of enzyme selectivity, substrate scope, activity and robustness. He critically reviews recent developments and case studies, takes a look at future applications in the field of organic synthesis, and concludes with lessons learned from previous experiments. |
| Carrier Form: | 1 online resource |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9783527655465 3527655468 9783527655496 3527655492 9783527655489 3527655484 |
| Index Number: | TP248 |
| CLC: | Q55 |
| Contents: | Introduction to Directed Evolution -- Selection versus Screening in Directed Evolution -- Gene Mutagenesis Methods -- Strategies for Applying Gene Mutagenesis Methods -- Selected Examples of Directed Evolution of Enzymes with Emphasis on Stereo- and Regioselectivity, Substrate Scope, and/or Activity -- Directed Evolution of Enzyme Robustness -- Directed Evolution of Promiscuity: Artificial Enzymes as Catalysts in Organic Chemistry -- Learning from Directed Evolution. |