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Transgenic animal technology : a laboratory handbook /

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions, as well as to make practical applications to production agriculture. This well-illustrated handbook covers the technical a...

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Bibliographic Details
Corporate Authors: Elsevier Science & Technology.
Group Author: Pinkert, Carl A. (Editor)
Published: Academic Press,
Publisher Address: San Diego :
Publication Dates: 1994.
Literature type: eBook
Language: English
Subjects:
Transgenic animals > Laboratory manuals.
Animal genetic engineering > Laboratory manuals.
Animals, Genetically Modified.
Genetic engineering.
NATURE > Mammals. > Mammals.
SCIENCE > Mammals. > Mammals. > Mammals.
Animal genetic engineering.
Transgenic animals.
Transgene organismen.
Technologie.
Gentechnologie.
Gentransfer.
Methode.
Tiere.
Transgene Tiere.
Animals > Genetics
Laboratory Manuals.
Electronic books.
Online Access: http://www.sciencedirect.com/science/book/9780125571654
Summary: Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions, as well as to make practical applications to production agriculture. This well-illustrated handbook covers the technical aspects of gene transfer in animals--from molecular methods to whole animal considerations--for the important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. Key Features * Contains a comprehensive collection of transgenic animal and gene transfer methods * Discusses background and introduction to techniques and animal systems * Teaches practical step-by-step protocols * Describes mouse model systems * Covers laboratory and domestic animal species, including: * Rabbits and rats * Poultry and fish * Swine and ruminants * Illustrated with diagrams and photographs * Includes useful tables and charts.
Carrier Form: 1 online resource (xi, 364 pages) : illustrations
Bibliography: Includes bibliographical references and index.
ISBN: 9780323137836
0323137830
Index Number: QH442
CLC: Q953-33
Contents: Introduction to transgenic animals / Carl A. Pinkert -- Transgenic animal production focusing on the mouse model -- DNA microinjection and transgenic animal production / H. Greg Polites and Carl A. Pinkert -- Factors affecting transgenic animal production / Paul A. Overbeek -- Gene transfer in embryonic stem cells / Thomas Doetschman -- Retrovirus-mediated gene transfer / Philip A. Wood -- Gene transfer technology: alternative techniques and applications / Glenn M. Monastersky -- Molecular approaches involved in mammalian gene transfer: analysis of transgene integration / Brad T. Tinkle, Charles J. Bieberich and Gilbert Jay -- Molecular approaches involved in mammalian gene transfer: evaluation of transgene expression / Charles J. Bieberich, Lien Ngo and Gilbert Jay -- Introduction of transgenic laboratory and domestic animal species -- Production of transgenic rats and rabbits / James M. Robl and Jan K. Heideman -- Production of transgenic poultry and fish / Linda C. Cioffi, John J. Kopchick and Howard Y. Chen -- Production of transgenic swine / Michael J. Martin and Carl A. Pinkert.
(cont) Production of transgenic ruminants / Caird E. Rexroad, Jr. and Harold W. Hawk.

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