Screen LibraryTransgenic animal technology : a laboratory handbook /
Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal cons...
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| Published: |
Academic Press,
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| Publisher Address: | Amsterdam ; Boston : |
| Publication Dates: | 2002. |
| Literature type: | eBook |
| Language: | English |
| Edition: | Second edition. |
| Subjects: | |
| Online Access: |
http://www.sciencedirect.com/science/book/9780080574806 |
| Summary: |
Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems. Contains a comprehensive collection of transgenic animal and gene transfer methods Discusses background and introduction to techniques and animal systems Teaches practical step-by-step protocols New section on analysis. |
| Carrier Form: | 1 online resource (xv, 618 pages :) : illustrations |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9780080574806 0080574807 |
| Index Number: | QH442 |
| CLC: | Q953-33 |
| Contents: |
Front Cover; Transgenic Animal Technology: A Laboratory Handbook; Copyright Page; Table of Contents; Contributors; Preface; PART 1: Overview; Chapter 1. Introduction to Transgenic Animal Technology; I. Introduction; II. Historical Background; III. Applications and Overview of Text; References; PART 2: Transgenic Animal Production Focusing on the Mouse Model; Chapter 2. DNA Microinjection and Transgenic Animal Production; I. Introduction; II. General Methods; III. Summary; Acknowledgments; References; Appendix; Chapter 3. Factors Affecting Transgenic Animal Production; I. Introduction. II. Getting StartedIII. Troubleshooting; IV. Transgenic Phenomenology; V. Summary; Acknowledgments; References; Chapter 4. Gene Targeting in Embryonic Stem Cells:; I. Stem Cells of the Embryo; II. Gene Targeting in Embryonic Stem Cells; III. Methods; Acknowledgments; References; Chapter 5. Gene Targeting in Embryonic Stem Cells:; I. Introduction; II. Conditional Technologies; III. Cre-loxP System Review, Development and Testing; IV. Cre-loxP and Transgenic Models; V. Methods; VI. Summary; Acknowledgments; References; Chapter 6. Retrovirus-Mediated Gene Transfer Teoan Kim. I. Introduction and DiscussionII. Methods; Acknowledgment; References; Chapter 7. Nuclear Transfer Technologies Yukio Tsunoda and Yoko Kato; I. Introduction and Discussion; II. Materials and Equipment; III. Preparation of Microtools; IV. Nuclear Transfer of Preimplantation Embryos; V. Nuclear Transfer of Embryonic Stem Cells and Somatic Cells; Acknowledgment; References; PART 3: Production of Transgenic Laboratory and Domestic Animal Species; Chapter 8. Production of Transgenic Rats; I. Introduction; II. Importance of Rats; III. Contributions from Transgenic Rats; IV. Production. V. ConclusionsReferences; Chapter 9. Production of Transgenic Rabbits; I. Applications of Transgenic Rabbits; II. Manipulation of Rabbit Embryos for Production of Transgenics; III. Materials and Methods; References; Chapter 10. Production of Transgenic Fish; I. Introduction; II. Discussion; III. Methods of Gene Transfer; IV. Establishing and Maintaining Transgenic Lineages; V. Care and Containment of Transgenic Fish; VI. Future of the Transgenic Fish; Acknowledgments; References; Chapter 11. Production of Transgenic Poultry; I. Introduction and Discussion; II. Methods; III. Summary. |