Screen LibraryThe surfaceome : methods and protocols /
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| Group Author: | ; |
|---|---|
| Published: |
Humana Press,
|
| Publisher Address: | New York, NY : |
| Publication Dates: | [2018] |
| Literature type: | Book |
| Language: | English |
| Series: |
Methods in molecular biology,
1722 Springer protocols |
| Subjects: | |
| Carrier Form: | xi, 347 pages : illustrations (some color), forms ; 26 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781493975518 (hardcover : alkaline paper) : 149397551X (hardcover : alkaline paper) 9781493975532 (eBook) 1493975536 (eBook) |
| Index Number: | QH634 |
| CLC: | Q251 |
| Call Number: | Q251/S961 |
| Contents: |
Surfaceome analysis protocol for the identification of novel ordetella pertussis antigens / "Shaving" live bacterial cells with proteases for proteomic analysis of surface proteins / Methods for mapping the extracellular and membrane proteome in the avian embryo, and identification of putative vascular targets or endothelial genes / Mass spectrometry-based identification of extracellular domains of cell surface N-glycoproteins : defining the accessible surfaceome for immunophenotyping stem cells and their derivatives / Application of higher density iron oxide nanoparticle pellicles to enrich the plasma membrane and its proteome from cells in suspension / Proteomic profiling of secreted proteins, exosomes, and microvesicles in cell culture conditioned media / Cloning, expression, and purification of the glycosylated transmembrane protein, cation-dependent mannose 6-phosphate receptor, from Sf9 cells using the baculovirus system / Bispecific antibody armed T cells to target cancer cells / Immunophenotyping of live human pluripotent stem cells by flow cytometry / Detecting cell surface expression of the G protein-coupled receptor CXCR4 / NaV channels : assaying biosynthesis, trafficking, function / High-content electrophysiological analysis of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) / Methods for evaluation of vascular endothelial cell function with transient receptor potential (TRP) channel drugs / Methods to study the signal transduction of the surface receptor tyrosine kinase TrkB in neurons / Polarized human retinal pigment epithelium exhibits distinct surface proteome on apical and basal plasma membranes / Extracellular matrix molecule-based capture of mesenchymal stromal cells under flow / Generation of induced pluripotent stem cells from patients with COL3A1 mutations and differentiation to smooth muscle cells for ECM-surfaceome analyses / Fabrication and mechanical properties measurements of 3D microtissues for the study of cell-matrix interactions / Discovery of surface target proteins linking drugs, molecular markers, gene regulation, protein networks, and disease by using a web-based platform targets-search / |