Screen LibraryProtein complex assembly : methods and protocols /
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| Group Author: | |
|---|---|
| Published: |
Humana Press : Springer,
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| Publisher Address: | New York, NY : |
| Publication Dates: | [2018] |
| Literature type: | Book |
| Language: | English |
| Series: |
Methods in molecular biology,
1764 Springer protocols, |
| Subjects: | |
| Carrier Form: | xv, 506 pages : illustrations (some color) ; 27 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781493977581 149397758X |
| Index Number: | QP551 |
| CLC: | Q51 |
| Call Number: | Q51/P967-46 |
| Contents: |
Experimental characterization of protein complex structure, dynamics, and assembly / High-throughput electron cryo-tomography of protein complexes and their assembly / Preparation of tunable microchips to visualize native protein complexes for single-particle electron microscopy / Time-resolved cryo-electron microscopy using a microfluidic chip / Characterizing protein-protein interactions using solution NMR spectroscopy / Reconstitution of isotopically labeled ribosomal protein L29 in the 50S large ribosomal subunit for solution-statea and solid-state NMR / Characterizing protein-protein interactions using deep sequencing coupled to yeast surface display / Structurally guided in vivo crosslinking / Characterizing intact macromolecular complexes using native mass spectrometry / Hydrogen-deuterium exchange mass spectrometry to study protein complexes / Structural analysis of protein complexes by cross-linking and mass spectrometry / Global characterization of protein complexes by biochemical purification-mass spectrometry (BP/MS) / Proteomic profiling of integrin adhesion complex assembly / Dual-color and 3D super-resolution microscopy of multi-protein assemblies / Correlative 3D structured illumination microscopy and single-molecule localization microscopy for imaging cancer invasion / Observing the assembly of protein complexes in living eukaryotic cells in super-resolution using refSOFI / Detecting purinosome metabolon formation with fluorescence microscopy / Analysis of bacterial pilus assembly by shearing and immunofluorescence microscopy / Expression, purification, and assembly of archaellum subcomplexes of Sulfolobus acidocaldarius / Unstable protein purification through the formation of stable complexes / Expressing multi-subunit complexes using biGBac / Computational modelling of protein complex structure and assembly / Inferring and using protein quaternary structure information from crystallographic data / Searching and extracting data from the EMBL-EBI complex portal / Automated computational inference of multi-protein assemblies from biochemical co-purification data / Multiscale computational model for simulating the kinetics of protein complex assembly / Flexible protein-protein docking with SwarmDock / Protein-protein docking using evolutionary information / Modeling structure and dynamics of protein complexes with SAXS profiles / Modeling the structure of helical assemblies with experimental constraints in rosetta / Selecting conformational ensembles using residual electron and anomalous density (READ) / |