Screen LibraryIn vitro mutagenesis : methods and protocols /
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| Group Author: | |
|---|---|
| Published: |
Humana Press,
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| Publisher Address: | New York : |
| Publication Dates: | [2017] |
| Literature type: | Book |
| Language: | English |
| Series: |
Methods in molecular biology,
1498 Springer protocols |
| Subjects: | |
| Item Description: | "Published In: United States, 06 October 2016" --Distributor's website. |
| Carrier Form: | xv, 511 pages : illustrations (some color) ; 26 cm. |
| Bibliography: | Includes bibliographical references and index. |
| ISBN: |
9781493964703 1493964704 |
| Index Number: | QH465 |
| CLC: | Q754 |
| Call Number: | Q754/I358 |
| Contents: |
Design and validation of CRISPR/Cas9 systems for targeted gene modification in induced pluripotent stem cells / Mutagenesis and genome engineering of epstein-barr virus in cultured human cells by CRISPR/Cas9 / Use of CRISPR/Cas genome editing technology for targeted mutagenesis in rice / All-in-one CRISPR-Cas9/FokI-dCas9 vector-mediated multiplex genome engineering in cultured cells / CRISPR/Cas9-mediated mutagenesis of human pluripotent stem cells in defined xeno-free E8 medium / Development of CRISPR/Cas9 for efficient genome editing in Toxoplasma gondii / Generation of stable knockout mammalian cells by TALEN-mediated locus-specific gene editing / Efficient generation of gene-modified mice by haploid embryonic stem cell-mediated semi-cloned technology / Insertion of group II intron-based ribozyme switches into homing endonuclease genes / Generating a genome editing nuclease for targeted mutagenesis in human cells / Use of group II intron technology for targeted mutagenesis in Chlamydia trachomatis / In silico approaches to identify mutagenesis targets to probe and alter protein-cofactor and protein-protein functional relationships / In silico prediction of deleteriousness for nonsynonymous and splice-altering single nucleotide variants in the human genome / In silico methods for analyzing mutagenesis targets / Methods for detecting critical residues in proteins / Method for bioinformatic analysis of transposon insertion sequencing (INSeq) results for identification of microbial fitness determinants / Application of in vitro transposon mutagenesis to erythromycin strain improvement in Saccharopolyspora erythraea / Engineering gram-negative microbial cell factories using transposon vectors / PERMutation using transposase engineering (PERMUTE) : a simple approach for constructing Circularly permuted protein libraries / Transposon insertion mutagenesis for archaeal gene discovery / Genome-wide transposon mutagenesis in Mycobacterium tuberculosis and Mycobacterium smegmatis / Multiple site-directed and saturation mutagenesis by the patch cloning method / Seamless ligation cloning extract (slice) method using cell lysates from laboratory Escherichia coli strains and its application to slip site-directed mutagenesis / Facile site-directed mutagenesis of large constructs using gibson isothermal DNA assembly / Revised mechanism and improved efficiency of the QuikChange site-directed mutagenesis method / In vitro single-primer site-directed mutagenesis method for use in biotechnology / Use of megaprimer and overlapping extension PCR (OE-PCR) to mutagenize and enhance cyclodextrin glucosyltransferase (CGTase) function / Step-by-step in vitro mutagenesis : lessons from fucose-binding lectin PA-IIL / Analytical methods for assessing the effects of site-directed mutagenesis on protein-cofactor and protein-protein functional relationships / Biochemical and biophysical methods to examine the effects of site-directed mutagenesis on enzymatic activities and interprotein interactions / Use of random and site-directed mutagenesis to probe protein structure-function relationships : applied techniques in the study of Helicobacter pylori / Novel random mutagenesis method for directed evolution / Random mutagenesis by error-prone polymerase chain reaction using a heavy water solvent / Development and use of a novel random mutagenesis method : in situ error-prone PCR (is -epPCR) / |